骨成型蛋白7(BMP7)活性蛋白
Active Bone Morphogenetic Protein 7 (BMP7)
OP1; Osteogenic Protein-1; Eptotermin alfa
- 編號UAPA799Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點8.5
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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SDS-PAGE圖
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Figure. Western Blot;
Sample: Recombinant BMP7, Mouse.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
BMP7 (Bone morphogenetic protein 7), which belongs to the TGF-β superfamily, is a signaling molecule with the ability to promote bone formation. BMP7 has also been implicated in various types of cancer, including breast cancer. It has been reported that BMP7 treatment induced cell growth promotion of MDA-MB-231 breast cancer line. To test the effect of BMP7 on cell proliferation,?MDA-MB-231 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM?prior to the addition of various concentrations of BMP7. After incubated for 72h,?cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C.
The dose-effect curve of BMP7 was shown in Figure 2. It was obvious that BMP7 significantly promoted cell proliferation of MDA-MB-231 cells. The ED50 for this effect is typically 3.483 to 9.017 ng/mL.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA799Mu01 | 骨成型蛋白7(BMP7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA799Mu01 | 骨成型蛋白7(BMP7)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA799Mu02 | 骨成型蛋白7(BMP7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA799Mu01 | 骨成型蛋白7(BMP7)多克隆抗體 | IHC |
| UPAA799Mu02 | 骨成型蛋白7(BMP7)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA799Mu71 | 骨成型蛋白7(BMP7)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA799Mu21 | 骨成型蛋白7(BMP7)單克隆抗體 | WB; IHC; ICC; IP. |
| ULAA799Mu72 | 骨成型蛋白7(BMP7)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA799Mu | 骨成型蛋白7(BMP7)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA799Mu | 骨成型蛋白7(BMP7)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA799Mu01 | 骨成型蛋白7(BMP7)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA799Mu01 | 骨成型蛋白7(BMP7)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| BioMed Research International | Serum Heme Oxygenase-1 and BMP-7 Are Potential Biomarkers for Bone Metabolism in Patients with Rheumatoid Arthritis and Ankylosing Spondylitis [Pubmed:27314037] |
| ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats [Pubmed: 32004919] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Temporal TGF-β Supergene Family Signalling Cues Modulating Tissue Morphogenesis: Chondrogenesis within a Muscle Tissue Model? [Pubmed: 32660137] |
