成纖維細胞生長因子18(FGF18)活性蛋白
Active Fibroblast Growth Factor 18 (FGF18)
ZFGF5
- 編號UAPC907Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點10.7
- 應用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure . Gene Sequencing (extract)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Fibroblast Growth Factor-18 (FGF18) is a trophic factor for mature chondrocytes
and their progenitors. It has been reported to have significant anabolic effects on
cartilage. FGF18 plays a central role in skeletal growth and
development.Besides,Fibroblast Growth Factor Receptor 3 (FGFR3) has been
identified as an interactor of FGF18, thus a binding ELISA assay was conducted
to detect the interaction of recombinant human FGF18 and recombinant human
FGFR3. Briefly, FGF18 were diluted serially in PBS, with 0.01% BSA (pH 7.4).
Duplicate samples of 100μl were then transferred to FGFR3-coated microtiter
wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated
for 1h with anti-FGF18 pAb, then aspirated and washed 3 times. After incubation
with HRP labelled secondary antibody, wells were aspirated and washed 3 times.
With the addition of substrate solution, wells were incubated 15-25 minutes at
37℃. Finally, add 50μl stop solution to the wells and read at 450nm immediately.
The binding activity of FGF18 and FGFR3 was shown in Figure 1, and this effect
was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| UEPC907Hu62 | 成纖維細胞生長因子18(FGF18)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPC907Hu02 | 成纖維細胞生長因子18(FGF18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC907Hu02 | 成纖維細胞生長因子18(FGF18)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPC907Hu01 | 成纖維細胞生長因子18(FGF18)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPC907Hu01 | 成纖維細胞生長因子18(FGF18)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAC907Hu01 | 成纖維細胞生長因子18(FGF18)多克隆抗體 | WB; IHC; ICC; IP. |
| UMAC907Hu22 | 成纖維細胞生長因子18(FGF18)單克隆抗體 | WB; IHC; ICC; IP. |
| USEC907Hu | 成纖維細胞生長因子18(FGF18)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEC907Hu | 成纖維細胞生長因子18(FGF18)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCC907Hu | 成纖維細胞生長因子18(FGF18)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| UCCC907Hu | 成纖維細胞生長因子18(FGF18)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMC907Hu | 成纖維細胞生長因子18(FGF18)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Pesqui. Bras. Odontopediatria Clín. Integr | Human Umbilical Cord Mesenchymal Stem Cells Over Platelet Rich Fibrin Scaffold for Mandibular Cartilage Defects Regenerative Medicine [] |
| J Exp Clin Cancer Res | HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway [Pubmed:35277183] |
