顆粒酶M(GZMM)活性蛋白
Active Granzyme M (GZMM)
GZM-M; LMET1; MET1; Hu-Met-1; Met-ase; Lymphocyte Met Ase 1; Met-1 serine protease; Natural killer cell granular protease
- 編號UAPA431Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 90%
- 等電點10.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant GZMM, Human.
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets a-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Human granzyme M is synthesized as a precursor (264?residues) with a signal peptide (residues 1 23), a propeptide (residues 24-25) and a mature chain (residues 26-257). The purified recombinant human Granzyme M consists of residues 26 to 257 which activity was measured by its ability to cleaves a thioester substrate Z-Lys-SBzl?HCl. The reaction was performed in 0.05 M Tris, 0.15 M NaCl, 0.01% Triton X-100, pH 8.0 (assay buffer), initiated by addition 50 μL of various concentrations of GZMM (diluted by assay buffer) to 50 μL of 1.2 mM substrate and DTNB mixture. The final well serves as a negative control with no GZMM, replace with 50 μL assay buffer. Incubated at 25℃ for 5min, then read at a wavelength of 405 nm. The specific activity of recombinant human Granzyme M is >80 pmol/min/μg.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA431Hu01 | 顆粒酶M(GZMM)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA431Hu01 | 顆粒酶M(GZMM)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA431Hu01 | 顆粒酶M(GZMM)多克隆抗體 | WB; IHC |
| ULAA431Hu71 | 顆粒酶M(GZMM)多克隆抗體(生物素標(biāo)記) | WB |
| ULAA431Hu81 | 顆粒酶M(GZMM)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAA431Hu23 | 顆粒酶M(GZMM)單克隆抗體 | WB |
| UMAA431Hu22 | 顆粒酶M(GZMM)單克隆抗體 | IHC |
| USEA431Hu | 顆粒酶M(GZMM)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Clinical Immunology | Elevated granzyme M-expressing lymphocytes during cytomegalovirus latency and reactivation after allogeneic stem cell transplantation [ScienceDirect: S1521661613002970] |
| Immunobiology. | Granzyme M and K release in human experimental endotoxemia [science:S0171298516300225] |
