心型脂肪酸結(jié)合蛋白(H-FABP)活性蛋白
Active Heart-type Fatty Acid Binding Protein (H-FABP)
MDGI; FABP11; H-FABP; O-FABP; Hfabp; FABP3; Fatty Acid Binding Protein 3, Muscle And Heart; Mammary Derived Growth Inhibitor; Fatty Acid Binding Protein 11; Muscle fatty acid-binding protein; Heart-ty
- 編號UAPB243Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 95%
- 等電點6.6
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實驗
Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) also known as mammary-derived growth inhibitor is a protein that in humans is encoded by the FABP3 gene. FABP3 is a small cytoplasmic protein (15kDa) released from cardiac myocytes following an ischemic episode. It is involved in active fatty acid metabolism where it transports fatty acids from the cell membrane to mitochondria for oxidation. Besides, Fatty Acid Binding Protein 3, Muscle And Heart (FABP3) has been identified as an interactor of SOD1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FABP3 and recombinant human SOD1. Briefly, FABP3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to SOD1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-FABP3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of FABP3 and SOD1 was shown in Figure 1, and this effect was in a dose dependent manner.
Figure. The binding activity of FABP3 with SOD1.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB243Hu01 | 心型脂肪酸結(jié)合蛋白(H-FABP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB243Hu01 | 心型脂肪酸結(jié)合蛋白(H-FABP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPB243Hu61 | 心型脂肪酸結(jié)合蛋白(H-FABP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB243Hu01 | 心型脂肪酸結(jié)合蛋白(H-FABP)多克隆抗體 | WB; IHC; ICC/IF; FCM |
| ULAB243Hu81 | 心型脂肪酸結(jié)合蛋白(H-FABP)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAB243Hu71 | 心型脂肪酸結(jié)合蛋白(H-FABP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAB243Hu26 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | WB |
| UMAB243Hu23 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | WB |
| UMAB243Hu24 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | WB |
| UMAB243Hu25 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | IHC |
| UMAB243Hu27 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | IHC |
| UMAB243Hu21 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB243Hu22 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | WB; IHC; ICC/IF |
| UMAB243Hu29 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | WB |
| UFAB243Hu02 | 抗心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體 | FCM |
| ULAB243Hu72 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體(生物素標(biāo)記) | WB |
| ULAB243Hu82 | 心型脂肪酸結(jié)合蛋白(H-FABP)單克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UHEB243Hu | 心型脂肪酸結(jié)合蛋白(H-FABP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEB243Hu | 心型脂肪酸結(jié)合蛋白(H-FABP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB243Hu | 心型脂肪酸結(jié)合蛋白(H-FABP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB243Hu01 | 心型脂肪酸結(jié)合蛋白(H-FABP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Molecular Medicine Reports | Protective effects of N (2)?L?alanyl?L?glutamine mediated by the JAK2/STAT3 signaling pathway on myocardial ischemia reperfusion [Pubmed:29393473] |
| Oncology?Letters | Hydrogen?rich solution attenuates myocardial injury caused by cardiopulmonary bypass in rats via the Janus?activated kinase 2/signal transducer and activator of?… [Pubmed:29928398] |
| FASEB?Journal | Signature-oriented investigation of the efficacy of multicomponent drugs against heart failure [Pubmed: 30230922] |
