干擾素α(IFNa)活性蛋白
Active Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- 編號(hào)UAPA033Mu61
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)9.1
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 10μg50μg 200μg 1mg 5mg
- 價(jià)格 ¥ 1080 ¥ 2700 ¥ 5400 ¥ 16200 ¥ 40500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Gene sequencing
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Figure. SDS-PAGE
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Figure. Western Blot; Sample: Recombinant IFNa, Mouse.
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
The Interferon-alpha?(IFNa) proteins belong to type I interferons (IFNs) which a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins produced by leukocytes are also known as leukocyte interferon. They are mainly involved in innate immune response against viral infection. Besides, Interferon-alpha?13 (IFNA13) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse IFNa and recombinant mouse IFNA13. Briefly, IFNA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNA13-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IFNapAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNA13 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過(guò)一個(gè)月，-80°C不超過(guò)12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA033Mu61 | 干擾素α(IFNa)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA033Mu01 | 干擾素α(IFNa)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA033Mu02 | 干擾素α(IFNa)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA033Mu61 | 干擾素α(IFNa)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA033Mu01 | 干擾素α(IFNa)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA033Mu06 | 干擾素α(IFNa)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA033Mu01 | 干擾素α(IFNa)多克隆抗體 | IHC |
| UPAA033Mu02 | 干擾素α(IFNa)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA033Mu71 | 干擾素α(IFNa)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAA033Mu21 | 干擾素α(IFNa)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA033Mu22 | 干擾素α(IFNa)單克隆抗體 | WB; IHC; ICC; IP. |
| ULAA033Mu72 | 干擾素α(IFNa)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAA033Mu76 | 干擾素α(IFNa)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA033Mu | 干擾素α(IFNa)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA033Mu | 干擾素α(IFNa)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA033Mu | 干擾素α(IFNa)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA033Mu | 干擾素α(IFNa)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA033Mu | 干擾素α(IFNa)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA033Mu01 | 干擾素α(IFNa)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Animal and Veterinary Advances | Tryptophan Stimulates Immune Response in Broiler Chickens Challenged with Infectious Bursal Disease Vaccine [Medwell: 2010610616] |
| Journal of Animal and Veterinary Advances | Immunostimulatory Effects of Arginine in Broiler Chickens Challenged with Vaccine Strain of Infectious Bursal Disease Virus [Medwell: 2010594600] |
| Ann Rheum Dis. | The cathelicidins LL-37 and rCRAMP are associated with pathogenic events of arthritis in humans and rats [PubMed: 23172753] |
| Nanomedicine | Efficient intracellular delivery of siRNA with a safe multitargeted lipid-based nanoplatform [PubMed: 23394132] |
| International journal of experimental pathology | Experimental rat model for alcohol-induced osteonecrosis of the femoral head [PubMed: PMC3781777] |
| Antiviral Research | A recombinant adenovirus bicistronically expressing porcine interferon-α and interferon-γ enhances antiviral effects against foot-and-mouth disease virus [ScienceDirect: S0166354214000266] |
| British Journal of Pharmacology | Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells [Onlinelibrary: bph.12440] |
| BMC Cancer | High efficiency of alphaviral gene transfer in combination with 5-fluorouracil in a mouse mammary tumor model [Pubmed:24950740] |
| PLoS One. | Respiratory Mechanics and Plasma Levels of Tumor Necrosis Factor Alpha and Interleukin 6 Are Affected by Gas Humidification during Mechanical Ventilation in Dogs [Pubmed:25036811] |
| Veterinary Research | Infection of equine monocyte-derived macrophages with an attenuated equine infectious anemia virus (EIAV) strain induces a strong resistance to the infection by a virulent EIAV strain [Pubmed:25106750] |
| Asian Pac J Trop Med | Effect of SecinH3 on lung injury induced by sepsis of rats [PubMed: 26706678] |
| PLoS?One | siRNA Targeting the 2A pro Genomic Region Prevents Enterovirus 71 Replication In Vitro [Pubmed:26886455] |
| Plos One | Sulforaphane?Suppresses?Hepatitis?C?Virus?Replication?by?Up-ReCavia (Guinea pig )lating?Heme?Oxygenase-1?Expressionthrough?PI3K/Nrf2?Pathway. [Pubmed:27023634] |
| PLoS One. | Vaccination with recombinant Mycobacterium tuberculosis PknD attenuates bacterial dissemination to the brain in Cavia (Guinea pig )inea pigs. [Pubmed:23776655] |
| Veterinary microbiology | Impact of porcine reproductive and respiratory syndrome virus and porcine circovirus-2 infection on the potency of the classical swine fever vaccine (LOM strain). [pubmed:27599928] |
| International Journal of?Clinical?and Experimental Pathology | Host miR-146a promotes replication of human cytomegalovirus by suppressing type I IFN response in MRC-5 cells [files:ijcep0036479.pdf] |
| Scandinavian?Journal?of?Immunology | Serum?Proteomics?Analysis?in?Rats?of?Immunosuppression?Induced?by?Chronic?Stress. [pubmed:27384769] |
| Saudi Journal of Gastroenterology | Deoxyschizandrin?suppresses?dss-induced?ulcerative?colitis?in?mice. [pubmed:27976641] |
| Biochemical and Biophysical Research Communications | MicroRNA-548j inhibits type I interferon production by targeting ZBTB11 in patients with chronic hepatitis B [pubmed:28342861] |
| Virology | The down-regulation of casein kinase 1 alpha as a host defense response against infectious bursal disease virus infection [pubmed:28988058] |
| Antiviral Research | Coinjection of a vaccine and anti-viral agents can provide fast-acting protection from foot-and-mouth disease [pubmed:28454913] |
| Molecular Therapy. Methods & Clinical Development | Anti-high mobility group box 1 antibody ameliorates albuminuria in MRL/lprlupus-prone mice [pubmed:28649578] |
| Journal of Virology | Fucoidan from Fucus vesiculosus suppresses hepatitis B virus replication by enhancing extracellular signal-regulated Kinase activation. [pubmed:28915824] |
| Clinical?Immunology | Toll-like receptor 9 ligands increase type I interferon induced B-cell activating factor expression in chronic rhinosinusitis with nasal polyposis [Pubmed:30056130] |
| International?forum?of?allergy?& rhinology | Role of Toll‐like receptor 9 signaling on activation of nasal polyp–derived fibroblasts and its association with nasal polypogenesis [Pubmed:29901280] |
| LUPUS | Collagen triple helix repeat containing-1: a novel biomarker associated with disease activity in Systemic lupus erythematosus [Pubmed: 30336754] |
| Journal of?Pineal?Research | Role of melatonin in sleep deprivation‐induced intestinal barrier dysfunction in mice [Pubmed: 30929267] |
| Veterinary Immunology and Immunopathology | Differential expression of type I interferon mRNA and protein levels induced by virulent Marek's disease virus infection in chickens [] |
| international immunopharmacology | Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice [Pubmed: 31228813] |
| FASEB Journal | Scavenger receptor A impairs interferon response to HBV infection by limiting TRAF 3 ubiquitination through recruiting OTUB 1 [] |
| Transboundary and Emerging Diseases | Pathogenicity of porcine reproductive and respiratory syndrome virus (ORF5 RFLP 1–7–4 viruses) in China [Pubmed: 32187856] |
| archives of virology | A flagellin-adjuvanted inactivated porcine epidemic diarrhea virus (PEDV) vaccine provides enhanced immune protection against PEDV challenge in piglets [Pubmed: 32253616] |
| Autophagy | LDHB inhibition induces mitophagy and facilitates the progression of CSFV infection [Pubmed: 32924761] |
| THERIOGENOLOGY | Porcine soluble CD83 alleviates LPS-induced abortion in mice by promoting Th2 cytokine production, Treg cell generation and trophoblast invasion [Pubmed: 32810792] |
| Int Immunopharmacol | BNIP3 deletion ameliorated enterovirus 71 infection-induced hand, foot and mouth disease via inhibiting apoptosis, autophagy, and inflammation in mice [Pubmed: 32717566] |
| Molecular Medicine Reports | Aquaporin?4 deletion ameliorates enterovirus 71 infection in mice [Pubmed: 32582978] |
| Virulence | Determination of antiviral action of Long Non-coding RNA loc107051710 During Infectious Bursal Disease Virus Infection due to Enhancement of Interferon?… [] |
| РОССИЙСКАЯ СЕЛЬСКОХОЗЯЙСТВЕННАЯ НАУКА | ГОРМОНАЛЬНЫЙ И ЦИТОКИНОВЫЙ ПРОФИЛЬ КРОВИ МОЛОЧНЫХ КОРОВ В РАННИЙ ПЕРИОД ГЕСТАЦИИ [] |
| Communications Biology | Influenza virus neuraminidase regulates host CD8+ T-cell response in mice [33293641] |
| Animals | Concentration-Dependent Type 1 Interferon-Induced Regulation of MX1 and FABP3 in Bovine Endometrial Explants [33494350] |
| Int J Dermatol | IFN?\|á and TNF?\|á serum levels and their association with disease severity in Egyptian children and adults with alopecia areata [34008204] |
| Dig Dis Sci | Orexin A Suppresses the Expression of Exosomal PD-L1 in Colon Cancer and Promotes T Cell Activity by Inhibiting JAK2/STAT3 Signaling Pathway [34097168] |
| Clinical findings of Talaromyces marneffei infection among patients with anti-interferon-|? immunodeficiency: a prospective cohort study [34144700] | |
| ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | Chronic developmental exposure to low-dose ([C8mim][PF6]) induces neurotoxicity and behavioural abnormalities in rats [34555716] |
| Carbohydrate Polymers | The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides [34823813] |
| Poult Sci | Effects of different selenium sources on duodenum and jejunum tight junction network and growth performance of broilers in a model of fluorine-induced?… [Pubmed:35066382] |
| Veterinary Microbiology | Self-assembled BPIV3 nanoparticles can induce comprehensive immune responses and protection against BPIV3 challenge by inducing dendritic cell maturation in?… [Pubmed:35395543] |
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