干擾素α(IFNa)活性蛋白
Active Interferon Alpha (IFNa)
IFNA1; IFL; LeIF D; IFN; IFN-Alpha; IFNA13; IFN-A; IFNAP22; IFN-Alpha 1b; Interferon Alpha 1b; Interferon, Leukocytic; IFN, Leukocyte; Interferon alpha-D
- 編號UAPA033Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 緩沖液成份20mM Tris, 150mM NaCl緩沖液（pH8.0, 含有1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose和Proclin300）
- 性狀凍干粉
- 純度> 97%
- 等電點8.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. Gene Sequencing (Extract)
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Figure. SDS-PAGE
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Figure. Western Blot
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通過ISO 9001、ISO 13485質(zhì)量體系認證
活性實驗
Figure. The binding activity of IFNa with IFNa/bR1.
Interferon Alpha (IFNa) belongs to a large subgroup of interferon proteins that help regulate the activity of the immune system. The IFNa proteins are produced by leukocytes. They are mainly involved in innate immune response against viral infection. It is also made synthetically as medication in hairy cell leukemia. Besides, Interferon Alpha/Beta Receptor 1 (IFNa/bR1) has been identified as an interactor of IFNa, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat IFNa and recombinant rat IFNa/bR1. Briefly, IFNa were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IFNa/bR1-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-IFNa pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of IFNa and IFNa/bR1 was shown in Figure 1, and this effect was in a dose dependent manner.
用法
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個月，-80°C不超過12個月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA033Ra01 | 干擾素α(IFNa)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA033Ra61 | 干擾素α(IFNa)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA033Ra01 | 干擾素α(IFNa)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA033Ra61 | 干擾素α(IFNa)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA033Ra01 | 干擾素α(IFNa)多克隆抗體 | WB; FCM |
| UPAA033Ra06 | 干擾素α(IFNa)多克隆抗體 | WB; IHC; ICC; IP. |
| UFAA033Ra01 | 抗干擾素α(IFNa)多克隆抗體 | FCM |
| UMAA033Ra21 | 干擾素α(IFNa)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA033Ra26 | 干擾素α(IFNa)單克隆抗體 | IHC |
| USEA033Ra | 干擾素α(IFNa)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UIEA033Ra | 干擾素α(IFNa)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA033Ra | 干擾素α(IFNa)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA033Ra | 干擾素α(IFNa)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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