嗜中性粒細(xì)胞胞漿因子2(NCF2)活性蛋白
Active Neutrophil Cytosolic Factor 2 (NCF2)
NOXA2; p67phox; 65kDa, Chronic Granulomatous Disease,Autosomal 2; NADPH Oxidase Activator 2; 67 kDa neutrophil oxidase factor; Neutrophil NADPH oxidase factor 2
- 編號(hào)UAPA300Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)5.9
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Neutrophil Cytosolic Factor 2 (NCF2) ,as kown as as NCF-2, NOXA2, P67PHOX, and P67-PHOX， is a vital component of the NADPH oxidase complex in neutrophils, essential for immune defense against pathogens. Its dysfunction can lead to severe immune disorders like CGD.It has been reported that when the cell is properly stimulated, NCF2 binds to NOX1 and other necessary subunits such as p47phox and p22phox to form a complete NADPH oxidase complex.Thus a functional ELISA assay was conducted to detect the interaction of recombinant mouse NCF2 and recombinant human NOX1. Briefly, NCF2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 μl were then transferred to NOX1-coated microtiter wells and incubated for 1h at 37℃. Wells were washed with PBST and incubated for 1h with anti-NCF2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37℃, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50 μL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant mouse NCF2 and recombinant human NOX1 was shown in Figure 1, the EC50?for this effect is 0.05ug/mL.
用法
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
儲(chǔ)存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
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| 編號(hào) | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA300Mu01 | 嗜中性粒細(xì)胞胞漿因子2(NCF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA300Mu01 | 嗜中性粒細(xì)胞胞漿因子2(NCF2)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA300Mu01 | 嗜中性粒細(xì)胞胞漿因子2(NCF2)多克隆抗體 | WB |
