前腦啡肽(PENK)活性蛋白
Active Proenkephalin (PENK)
Preproenkephalin; Proenkephalin-A; Synenkephalin; Met-enkephalin; Met-enkephalin-Arg-Gly-Leu; Leu-enkephalin; Met-enkephalin-Arg-Phe
- 編號UAPD396Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 緩沖液成份PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
- 性狀凍干粉
- 純度> 95%
- 等電點(diǎn)5.3
- 應(yīng)用Cell?culture;?Activity?Assays.
- 下載 英文說明書 中文說明書
- 規(guī)格 10μg50μg 200μg 1mg 5mg
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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Figure. SDS-PAGE
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
活性實(shí)驗(yàn)
Proenkephalin (PENK) is a protein encoded by the PENK gene. It is initially synthesized as a preproprotein and undergoes proteolytic processing to generate multiple products. As PENK has the function of inhibit the migration cells，we measure the activity of recombinant mouse PENK by the ability of the protein to inhibit the migration of U2OS cells. Thus, chemotaxis?assay used 24-well microchemotaxis system was undertaken to detect the inhibition of chemotactic effect of recombinant mouse PENK on U2OS cell line. Briefly, U2OS cells were seeded into the upper chambers and PENK was added in lower chamber with a polycarbonate filter (8 μm pore size) used to separate the two compartments. After incubation at 37℃ with 5% CO2 for 2h, the filter was removed, then cells in low chamber were observed by inverted microscope at low magnification (×10) and the number of migrated cells were counted randomly (five fields for each filter). Result shows MIP-3α is able to induce migration of U2OS cells. The migrated U2OS cells in low chamber at low magnification (×10) were shown in Figure 1.
用法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
儲存
避免反復(fù)凍融。2-8°C不超過一個(gè)月，-80°C不超過12個(gè)月。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPD396Mu01 | 前腦啡肽(PENK)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPD396Mu02 | 前腦啡肽(PENK)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPD396Mu01 | 前腦啡肽(PENK)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAD396Mu01 | 前腦啡肽(PENK)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAD396Mu02 | 前腦啡肽(PENK)多克隆抗體 | WB; IHC; ICC; IP. |
| USED396Mu | 前腦啡肽(PENK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMD396Mu | 前腦啡肽(PENK)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
