半乳糖凝集素1(GAL1)多克隆抗體
Polyclonal Antibody to Galectin 1 (GAL1)
LGALS1; GBP; HLBP14; Galaptin; Lectin,Galactoside-Binding Soluble 1; 14 kDa laminin-binding protein; Lactose-binding lectin 1; Putative MAPK-activating protein PM12; S-Lac lectin
- 編號UPAA321Bo01
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA321Bo01-半乳糖凝集素1(GAL1)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度500μg/mL
- 且適物種Homo sapiens (Human，人), Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 340 ¥ 794 ¥ 1134 ¥ 2835 ¥ 11340
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Bovine Cardiac Muscle Tissue; Primary Ab: 20μg/ml Rabbit Anti-Bovine GAL1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Bovine Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine GAL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Bovine Lymph node Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Bovine GAL1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant GAL1, Bovine.
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Western Blot; Sample: Bovine Kidney lysate;
Primary Ab: 5μg/ml Rabbit Anti-Bovine GAL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Bovine Skeletal muscle lysate;
Primary Ab: 5μg/ml Rabbit Anti-Bovine GAL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Porcine Skeletal muscle lysate;
Primary Ab: 5μg/ml Rabbit Anti-Bovine GAL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Skeletal muscle lysate;
Primary Ab: 5μg/ml Rabbit Anti-Bovine GAL1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: K562 cell lysate; Lane2: Hela cell lysate; Lane3: C2C12 cell lysate;
Primary Ab: 0.5μg/ml Rabbit Anti-Bovine GAL1 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human MDA-MB-231 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-Bovine GAL1 Polyclonal Antibody (Catalog PAA321Bo01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對GAL1的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別GAL1。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA321Bo01 | 半乳糖凝集素1(GAL1)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA321Bo01 | 半乳糖凝集素1(GAL1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA321Bo01 | 半乳糖凝集素1(GAL1)多克隆抗體 | WB; IHC; FCM |
| ULAA321Bo81 | 半乳糖凝集素1(GAL1)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA321Bo71 | 半乳糖凝集素1(GAL1)多克隆抗體(生物素標(biāo)記) | WB |
| UMAA321Bo24 | 半乳糖凝集素1(GAL1)單克隆抗體 | WB |
| UMAA321Bo22 | 半乳糖凝集素1(GAL1)單克隆抗體 | WB |
| UMAA321Bo23 | 半乳糖凝集素1(GAL1)單克隆抗體 | WB |
| UMAA321Bo21 | 半乳糖凝集素1(GAL1)單克隆抗體 | WB |
| UFAA321Bo08 | 抗半乳糖凝集素1(GAL1)單克隆抗體 | FCM |
| UFAA321Bo06 | 抗半乳糖凝集素1(GAL1)單克隆抗體 | FCM |
| UFAA321Bo04 | 抗半乳糖凝集素1(GAL1)單克隆抗體 | FCM |
| UFAA321Bo02 | 抗半乳糖凝集素1(GAL1)單克隆抗體 | FCM |
| USEA321Bo | 半乳糖凝集素1(GAL1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA321Bo | 半乳糖凝集素1(GAL1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA321Bo01 | 半乳糖凝集素1(GAL1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| International Journal of Oncology | Galectin-1-mediated cell adhesion, invasion and cell death in human anaplastic large cell lymphoma: Regulatory roles of cell surface glycans [Pubmed:24589677] |
| Clinica Chimica Acta | Galectin-1 and galectin-3: plausible tumour markers for oral squamous cell carcinoma and suitable targets for screening high-risk population [Pubmed:25578395] |
| Journal of Perinatal Medicine | Cord blood galectin-1 and-3 concentrations in term pregnancies with normal restricted and increased fetal growth [PubMed: 24717333] |
| Biomaterials | Noninvasive small-animal imaging of galectin-1 upregulation for predicting tumor resistance to radiotherapy [Pubmed:29272764] |
| Advances in Experimental?Medicine?and?Biology | Molecules of Damage-Associated Patterns in Bronchoalveolar Lavage Fluid and Serum in Chronic Obstructive Pulmonary Disease [Pubmed:29429028] |
| RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY | Elevated level of Galectin-1 in bronchoalveolar lavage of patients with idiopathic pulmonary fibrosis [Pubmed: 31678459] |
| Eur J Nucl Med Mol Imaging | Galectin expression detected by 68Ga-galectracer PET as a predictive biomarker of radiotherapy resistance [Pubmed:35106644] |
| Medicine | Regulatory T cells, damage-associated molecular patterns, and myeloid-derived suppressor cells in bronchoalveolar lavage fluid interlinked with chronic obstructive?… [Pubmed:35687771] |
| In Vitro Bovine Embryo Production Using Medium's Supplemented with Recombinant Human Galectin-1 Buffered [] |
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