谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體
Polyclonal Antibody to Transglutaminase 2 (TGM2)
tTG; TG2; C Polypeptide,Protein-Glutamine-Gamma-Glutamyltransferase; Transglutaminase 2, Tissue; Transglutaminase C; Transglutaminase H
- 編號UPAB830Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPB830Hu01-谷氨酰胺轉(zhuǎn)胺酶2(TGM2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; ICC/IF
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 299 ¥ 697 ¥ 996 ¥ 2490 ¥ 9960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human MCF7 cell; Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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DAB staining on IHC-P;<br/>Sample: Human Uterus Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Placenta Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Lymph node Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Spleen Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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DAB staining on IHC-P;<br/>Sample: Human Uterus Tissue; <br/>Primary Ab: 20μg/ml Rabbit Anti-Human TGM2 Antibody<br/>Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Sample: Hela cell lysate Primary Ab: 0.3μg/ml Rabbit Anti-Human TGM2 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Mouse Placenta lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Hela cell lysate;
Lane 2: TGM2 knockout Hela cell lysate;
Predicted MW: 77kd
Observed MW: 77kd
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Rat Lung lysate; Lane2: Rat Placenta lysate; Lane3: HepG2 cell lysate; Lane4: K562 cell lysate; Lane5: Hela cell lysate
Primary Ab: 0.1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human MCF7 cell lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human TGM2 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對TGM2的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別TGM2。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體 | WB; IHC; ICC/IF |
| ULAB830Hu71 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)多克隆抗體(生物素標記) | WB |
| UMAB830Hu22 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)單克隆抗體 | WB; IHC; ICC; IP. |
| UMEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB830Hu | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
| UCGB830Hu01 | 谷氨酰胺轉(zhuǎn)胺酶2(TGM2)cDNA | PCR、蛋白表達等 |
參考文獻
| 雜志 | 參考文獻 |
| Connective Tissue Research | Tissue transglutaminase is involved in mechanical load–induced osteogenic differentiation of human ligamentum flavum cells [Pubmed:27115725] |
| American Journal of Respiratory Cell and Molecular Biology | ECM Crosslinking Enhances Fibroblast Growth and Protects Against Matrix Proteolysis in Lung Fibrosis. [pubmed:29053339] |
| 3 Biotech | Metformin attenuates TGF?β1?induced pulmonary fibrosis through inhibition of transglutaminase 2 and subsequent TGF?β pathways [Pubmed: 32550106] |
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