白蛋白(ALB)檢測試劑盒(化學(xué)發(fā)光免疫分析法)
CLIA Kit for Albumin (ALB)
Serum albumin
- 編號UCCB028Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍1.56-400ug/mL
- 靈敏度最小可檢測劑量小于等于0.52ug/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1764 ¥ 2520 ¥ 11340 ¥ 21420 ¥ 176400
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測白蛋白(ALB)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白蛋白(ALB)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 84-101 | 91 |
| EDTA plasma(n=5) | 81-104 | 99 |
| heparin plasma(n=5) | 96-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白蛋白(ALB)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白蛋白(ALB)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-103% | 81-96% | 97-105% | 83-91% |
| EDTA plasma(n=5) | 90-98% | 98-105% | 94-102% | 83-91% |
| heparin plasma(n=5) | 83-103% | 85-101% | 98-105% | 88-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加底物100µL，37°C孵育10分鐘；
7. 讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將白蛋白(ALB)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原（標(biāo)準(zhǔn)品或樣本），待測抗原與生物素標(biāo)記抗原對特異性抗體進行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，再次徹底洗滌后加入底物。加入底物后會產(chǎn)生輝光型光信號。發(fā)射光強度和樣品中的白蛋白(ALB)呈負相關(guān)。用化學(xué)發(fā)光儀測定相對光單位（RLU），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UNPB028Hu01 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UNPB028Hu02 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB028Hu01 | 白蛋白(ALB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPB028Hu51 | 白蛋白(ALB)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB028Hu07 | 白蛋白(ALB)多克隆抗體 | WB |
| UPAB028Hu05 | 白蛋白(ALB)多克隆抗體 | WB; IHC |
| UPAB028Hu51 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAB028Hu01 | 白蛋白(ALB)多克隆抗體 | WB |
| UPAB028Hu02 | 白蛋白(ALB)多克隆抗體 | WB,ICC/IF |
| ULAB028Hu71 | 白蛋白(ALB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| ULAB028Hu81 | 白蛋白(ALB)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| UMAB028Hu23 | 白蛋白(ALB)單克隆抗體 | WB |
| UMAB028Hu24 | 白蛋白(ALB)單克隆抗體 | WB; IHC |
| UMAB028Hu25 | 白蛋白(ALB)單克隆抗體 | WB; IHC |
| UMAB028Hu26 | 白蛋白(ALB)單克隆抗體 | WB; IHC |
| UMAB028Hu27 | 白蛋白(ALB)單克隆抗體 | WB; IHC |
| UMAB028Hu29 | 白蛋白(ALB)單克隆抗體 | WB |
| UMAB028Hu22 | 白蛋白(ALB)單克隆抗體 | WB; IHC |
| UMAB028Hu28 | 白蛋白(ALB)單克隆抗體 | IHC |
| UMAB028Hu21 | 白蛋白(ALB)單克隆抗體 | WB |
| UFAB028Hu02 | 抗白蛋白(ALB)單克隆抗體 | FCM |
| UHEB028Hu | 白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEB028Hu | 白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEB028Hu | 白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEB028Hu | 抗白蛋白抗體(Anti-ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| UACB028Hu | 抗白蛋白抗體(Anti-ALB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antibody Detection. |
| UCCB028Hu | 白蛋白(ALB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMB028Hu | 白蛋白(ALB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSB028Hu11 | 白蛋白(ALB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Journal of Developmental Origins of?Health?and?Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring [Pubmed:29582727] |
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