促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Corticotropin Releasing Factor (CRF)
CRH; Corticotropin Releasing Hormone; Corticoliberin
- 編號UKSA835Hu11
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、生物素標(biāo)記的競爭物、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma and other biological fluids.
- 可適用原理競爭抑制酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍12.35-1000pg/mL
- 可適靈敏度4.91pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 3402 ¥ 5670 ¥ 10206 ¥ 19845 ¥ 34020
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對促腎上皮質(zhì)激素釋放激素(CRH)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA835Hu04 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA835Hu03 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA835Hu01 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA835Hu63 | 促腎上皮質(zhì)激素釋放激素(CRF)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA835Hu02 | 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UCPA835Hu24 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA835Hu25 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA835Hu21 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA835Hu22 | 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UCPA835Hu33 | 促腎上皮質(zhì)激素釋放激素(CRF)鑰孔血藍(lán)蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| UPAA835Hu01 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | IHC |
| UPAA835Hu03 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA835Hu02 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | IHC |
| UPAA835Hu04 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | WB |
| UPAA835Hu07 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA835Hu08 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| UPAA835Hu09 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA835Hu81 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA835Hu71 | 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體(生物素標(biāo)記) | WB |
| UMAA835Hu22 | 促腎上皮質(zhì)激素釋放激素(CRF)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA835Hu21 | 促腎上皮質(zhì)激素釋放激素(CRF)單克隆抗體 | WB |
| UCEA835Hu | 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA835Hu | 促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA835Hu11 | 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Toxicology Letters | Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345] |
| Bipolar?Disord. | Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β? [Pubmed:24862833] |
| Peptides | Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411] |
| Ann Dermatol | Acute?Stress-Induced?Changes?in?Follicular?Dermal?Papilla?Cells?and?Mobilization?of?Mast?Cells:Implications?for?Hair?Growth. [pubmed:27746640] |
| Neuroscience | Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093] |
| Biomed Research International | Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation [pubmed:29082242] |
| Biochemical Pharmacology | Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991] |
| Frontiers in Pharmacology | Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?… [Pubmed:30038568] |
| Toxicology Letters | Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors [Pubmed:29807116] |
| Neuropsychiatric disease and treatment | The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic?… [Pubmed:29379286] |
| BMC?Veterinary?Research | Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?… [Pubmed:29540178] |
| Bioscience Research | Bioscience Research [] |
| FASEB Journal | Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice [Pubmed: 31431066] |
| Aging Cell | Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582] |
| Endocr J | Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress [33408312] |
| CRYOBIOLOGY | O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993] |
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