基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Matrix Metalloproteinase 3 (MMP3)
SL1; STMY; STMY1; STR1; Progelatinase; Stromelysin 1; Transin-1
- 編號UKSA101Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍31.2-2000pg/mL
- 可適靈敏度13.1pg/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 2419 ¥ 4032 ¥ 7258 ¥ 14112 ¥ 24192
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對基質(zhì)金屬蛋白酶3(MMP3)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA101Hu62 | 基質(zhì)金屬蛋白酶3(MMP3)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA101Hu62 | 基質(zhì)金屬蛋白酶3(MMP3)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA101Hu01 | 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA101Hu02 | 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA101Hu04 | 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA101Hu03 | 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA101Hu61 | 基質(zhì)金屬蛋白酶3(MMP3)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA101Hu05 | 基質(zhì)金屬蛋白酶3(MMP3)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA101Hu02 | 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 | WB; IHC |
| UPAA101Hu01 | 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 | IHC |
| UPAA101Hu05 | 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 | IHC |
| UPAA101Hu03 | 基質(zhì)金屬蛋白酶3(MMP3)多克隆抗體 | WB; IHC |
| UMAA101Hu22 | 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體 | WB |
| UMAA101Hu23 | 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體 | WB; IHC; ICC; IP. |
| UMAA101Hu21 | 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體 | WB; IHC |
| ULAA101Hu72 | 基質(zhì)金屬蛋白酶3(MMP3)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| USEA101Hu | 基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USCA101Hu | 基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA101Hu | 基質(zhì)金屬蛋白酶3(MMP3)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA101Hu01 | 基質(zhì)金屬蛋白酶3(MMP3)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA101Hu01 | 基質(zhì)金屬蛋白酶3(MMP3)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Bone and Joint Surgery | Experimental osteoarthritis induced by surgical realignment of the patella in BALB/c mice [PubMed: 21119180] |
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| PLoS ONE | A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source] |
| China Pharmacy | Influence of Tongfengtai Granules on Inflammatory Factor in Acute Gouty Arthritis Model Rats [Source] |
| Thromb Haemost. | Analysis of the expression of nine secreted matrix metalloproteinases and their endogenous inhibitors in the brain of mice subjected to ischaemic stroke [Pubmed:24671655] |
| Connective Tissue Research | Metabolic and cytoprotective effects of in vivo peri-patellar hyaluronic acid injections in cultured tenocytes [Pubmed:25333747] |
| physiological research | The skin remodeling in type 1 diabetes and insulin resistance animal models [PubMed: 26047379] |
| Physiol Res | The skin remodeling in type 1 diabetes and insulin resistance animal models. [PubMed: 26047379] |
| Cellular & Molecular Immunology | Effect of ebosin on modulating interleukin-2&bgr;-induced inflammatory responses in rat fibroblast-like synoviocytes [PubMed: 25938977] |
| Biol Res | The role of MCP-1-CCR2 ligand-receptor axis in chondrocyte degradation and disease progress in knee osteoarthritis [PubMed: 26578310] |
| J Orthop Res. | Tetrandrine suppresses articular inflammatory response by inhibiting pro-inflammatory factors via NF-κB inactivation. [Pubmed:26748661] |
| Cellular?& Molecular Immunology | Effect of ebosin on modulating interleukin-1β-induced inflammatory responses in rat fibroblast-like synoviocytes [pubmed:25938977] |
| Journal of Orthopaedic?Research | Tetrandrine suppresses articular inflammatory response by inhibiting pro‐inflammatory factors via NF‐κB inactivation [pubmed:26748661] |
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| Molecular Medicine Reports | Icariin inhibits MMP?1, MMP?3 and MMP?13 expression through MAPK pathways in IL?1β?stimulated SW1353 chondrosarcoma cells [pubmed:28447732] |
| der Christian-Albrechts-Universit?t zu Kiel | Wirkung schonender Laserverfahren auf den Komplexaus retinalem Pigmentepithel, Bruch′scher Membranund Choroidea - m?gliche Therapieoption der frühen altersabh?ngigen Makuladegeneration [1133074014:34] |
| REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease [E-ISSN: 1695-7504] |
| REDVET - Revista electrónica de Veterinaria | Measurement of Metalloproteinase-3 levels in synovial fluid from horses as biomarker of joint disease - Medición de los niveles de Metaloproteinasa-3 en liquido sinovial comobiomarcador de enfermedad articular [ISSN 1695-7504] |
| Molecular Pharmacology | Dexamethasone Down-regulates Endothelin Receptors and Reduces Endothelin-induced Production of Matrix Metalloproteinases in Cultured Rat Astrocytes [10.1124/mol.116.107300] |
| Theranostics | Targeting intracellular MMPs efficiently inhibits tumor metastasis and angiogenesis [Pubmed:29774078] |
| Chinese Journal of Integrative Medicine | Electroacupuncture Delays Cartilage Degeneration by Modulating Nuclear Factor-κB Signaling Pathway [Pubmed: 30656598] |
| Effects of a hyperimmunized egg product on voluntary physical activity levels, serum inflammatory markers, and owner perception of joint pain of dogs with?… [] | |
| PLoS One | Co-culture of osteochondral explants and synovial membrane as in vitro model for osteoarthritis [Pubmed: 30939166] |
| ACS Biomaterials Science & Engineering | Fluorogenic Probe for Detecting Active Matrix Metalloproteinase-3 (MMP-3) in Plasma and Peripheral Blood Neutrophils to Indicate the Severity of Rheumatoid?… [] |
| World Journal of Pediatrics | Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis [Pubmed: 31641939] |
| International Journal of Molecular Sciences | Matrix Metalloproteinase-3 is Key Effector of TNF-α-Induced Collagen Degradation in Skin [Pubmed: 31652545] |
| JOURNAL OF CELLULAR BIOCHEMISTRY | Teneligliptin inhibits IL‐1β‐induced degradation of extracellular matrix in human chondrocytes [Pubmed: 32162384] |
| Bone Research | Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis [Pubmed: 32047705] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
| Alexandria Journal for Veterinary Sciences | The Clinicopathological Picture of Some Postpartum Disorders in Barki Ewes. [] |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats [] | |
| Food Funct | Undenatured type II collagen prevents and treats the osteoarthritis and motor function degradation in T2DM patients and db/db mice [33890588] |
| journal biomedicine translational research | Peanut (Arachis hypogaea) Shells Extract and Apis dorsata Honey Reduce Matrix Metalloproteinase-3 in Monosodium Iodoacetate-Induced Osteoarthritic??- [] |
| JOURNAL OF ANIMAL SCIENCE | Physical Activity Patterns of Free Living Dogs Diagnosed with Osteoarthritis [34216471] |
| Osteoarthritis Cartilage | A systematic review on the potential value of synovial fluid biomarkers to predict clinical outcomes in cartilage repair treatments [Pubmed:35618204] |
| Phytomedicine | Transcriptomic profiling revealed the role of apigenin-4′-O-α-L-rhamnoside in inhibiting the activation of rheumatoid arthritis fibroblast-like synoviocytes via MAPK?… [Pubmed:35660352] |
| Journal of Immunology Research | Ebosin Attenuates the Inflammatory Responses Induced by TNF-α through Inhibiting NF-κB and MAPK Pathways in Rat Fibroblast-Like Synoviocytes [Pubmed:35340587] |
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