血管生成素1(ANGPT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Angiopoietin 1 (ANGPT1)
ANG1; AGP1; AGPT; ANG-I
- 編號USEA008Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍2.34-150ng/mL
- 靈敏度最小可檢測劑量小于等于0.93ng/mL.
- 樣本類型Serum, plasma and other biological fluids.
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1512 ¥ 2160 ¥ 9720 ¥ 18360 ¥ 151200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測血管生成素1(ANGPT1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的血管生成素1(ANGPT1)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-105 | 97 |
| EDTA plasma(n=5) | 83-101 | 90 |
| heparin plasma(n=5) | 91-101 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管生成素1(ANGPT1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管生成素1(ANGPT1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 95-102% | 78-98% | 82-101% |
| EDTA plasma(n=5) | 83-97% | 99-105% | 88-95% | 79-92% |
| heparin plasma(n=5) | 93-101% | 78-101% | 79-92% | 79-98% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將血管生成素1(ANGPT1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的血管生成素1(ANGPT1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管生成素1(ANGPT1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管生成素1(ANGPT1)呈正相關(guān)。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應用(僅供研究使用，不用于臨床診斷！) |
| URPA008Ca01 | 血管生成素1(ANGPT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA008Ca02 | 血管生成素1(ANGPT1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA008Ca02 | 血管生成素1(ANGPT1)多克隆抗體 | WB |
| UPAA008Ca01 | 血管生成素1(ANGPT1)多克隆抗體 | WB; IHC; ICC; IP. |
| ULAA008Ca71 | 血管生成素1(ANGPT1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| USEA008Ca | 血管生成素1(ANGPT1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA008Ca | 血管生成素1(ANGPT1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Theriogenology | Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article] |
| J Atheroscler Thromb | High Mobility Group Box 1 Promotes Angiogenesis from Bone Marrow-derived Endothelial Progenitor Cells after Myocardial Infarction [PubMed: 25735431] |
| Archives of Biological Sciences | Diabetes-induced renal failure is associated with tissue inflammation and neutrophil gelatinase-associated lipocalin: Effects of resveratrol [publication:299492265_Diabetes-induced_renal_failu] |
| Am J Transl Res | Angiopoietin-1 facilitates recovery of hematopoiesis in radiated mice [Pubmed:27347310] |
| Clinical research | Increased plasma concentrations of interleukin 35 in patients with coronary artery disease [Journal:-19] |
| Turkish Journal of Medicl Sciences | A new angiogenesis prognostic index with VEGFA, PlGF, and angiopoietin 1 predicts survival in patients with advanced gastric cancer [pubmed:28425270] |
| Sci Rep.? | Flunarizine suppresses endothelial Angiopoietin-2 in a calcium - dependent fashion in sepsis. [pubmed:28276491] |
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| Free Patents online | RHEUMATOID ARTHRITIS RELATED BIOMARKER [20170350884] |
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| Clinical Significance of Nectin-4 Expression in Metastasis and Angiogenesis for Tumor Relapse [Doi: 10.4143/crt.2018.214] | |
| BIOLOGY?OF?REPRODUCTION | Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors [Pubmed: 30929911] |
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| Biochem Biophys Res Commun | Effects of interleukin-6 and tumor necrosis factor-α on the expression of angiogenic and collagenolytic factors in premature and mature adipocytes [Pubmed: 32800538] |
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