白介素13(IL13)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Interleukin 13 (IL13)
ALRH; BHR1; P600; IL-13; NC30
- 編號USEA060Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于5.9pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1512 ¥ 2160 ¥ 9720 ¥ 18360 ¥ 151200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測白介素13(IL13)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素13(IL13)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-94 | 89 |
| EDTA plasma(n=5) | 96-105 | 101 |
| heparin plasma(n=5) | 92-99 | 96 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素13(IL13)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素13(IL13)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-101% | 79-89% | 79-98% | 84-99% |
| EDTA plasma(n=5) | 80-101% | 91-102% | 87-96% | 99-105% |
| heparin plasma(n=5) | 96-103% | 94-101% | 97-105% | 85-92% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將白介素13(IL13)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的白介素13(IL13)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的白介素13(IL13)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的白介素13(IL13)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA060Ca01 | 白介素13(IL13)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA060Ca01 | 白介素13(IL13)多克隆抗體 | WB |
| UMAA060Ca21 | 白介素13(IL13)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA060Ca | 白介素13(IL13)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA060Ca | 白介素13(IL13)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Critical Care Medicine | Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage [PubMed: PMC3383056] |
| Int J Clin Exp Pathol | Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation [PubMed: 26722390] |
| Advances in Experimental Medicine and Biology? | Antiinflammatory Effect of N-Acetylcysteine Combined with Exogenous Surfactant in Meconium-Induced Lung Injury [Pubmed:27283193] |
| Molecular Immunology | Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by reCavia (Guinea pig )lating caveolin-1 through TLR2/MyD88/NF-??B in murine model of asthma [Pubmed:27289030] |
| Journal of Agricultural Biotechnology | Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells [10610] |
| BMC Veterinary Research | Lymphocytic, cytokine and transcriptomic profiles in peripheral blood of dogs with atopic dermatitis [pubmed:27553600] |
| Molecular?Immunology | Targeted inhibition of GATA-6 attenuates airway inflammation and remodeling by regulating caveolin-1 through TLR2/MyD88/NF-κB in murine model of asthma [pubmed:27289030] |
| Physiological?Research | Lung inflammatory and oxidative alterations after exogenous surfactant therapy fortified with budesonide in rabbit model of meconium aspiration syndrome. [pubmed:28006947] |
| Gut Pathog. | Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847] |
| Veterinary Immunology and Immunopathology | Cytokine levels in colostrum and in foals' serum pre- and post-suckling [pubmed:28242000] |
| Asian Pacific Society of Respirology | Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397] |
| Journal of Autoimmunity | Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003] |
| bulletin of experimental biology and medicine | Quantitative and Qualitative Characterization of Phagocytic Activity of Macrophages of Bone Marrow and Fetal Origin [Pubmed: 31183654] |
| life sciences | Melatonin biosynthesis restored by CpG oligodeoxynucleotides attenuates allergic airway inflammation via regulating NLRP3 inflammasome [Pubmed: 31738882] |
| Frontiers in Immunology | TLR2-Melatonin Feedback Loop Regulates the Activation of NLRP3 Inflammasome in Murine Allergic Airway Inflammation [Pubmed: 32117301] |
| Parasites & Vectors | Fasciola gigantica excretory-secretory products (FgESPs) modulate the differentiation and immune functions of buffalo dendritic cells through a mechanism?… [Pubmed: 32680553] |
| Biomedicine & Pharmacotherapy | Qingfei oral liquid alleviates airway hyperresponsiveness and mucus hypersecretion via TRPV1 signaling in RSV-infected asthmatic mice [Pubmed: 32521453] |
| Journal of Traditional Chinese Medical Sciences | Mahuang Xixin Fuzi Decoction protects against ovalbumin-induced allergic rhinitis by inhibiting type 2 innate lymphoid cells in mice [] |
| Traditional Medicine Research | Qingfei oral liquid downregulates TRPV1 expression to reduce airway inflammation and mucus hypersecretion injury caused by respiratory syncytial virus infection … [] |
| Applied materials today | Tanshinone IIA-loaded aligned microfibers facilitate stem cell recruitment and capillary formation by inducing M2 macrophage polarization [] |
| Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota [] | |
| Biomed Pharmacother | Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice [33706133] |
| BIOMATERIALS | Aligned microfiber-induced macrophage polarization to guide schwann-cell-enabled peripheral nerve regeneration [33813259] |
| PLoS One | Beneficial impact of cathelicidin on hypersensitivity pneumonitis treatment?aIn vivo studies [33999928] |
| Anti-inflammatory activity of non-selective PDE inhibitor aminophylline on the lung tissue and respiratory parameters in animal model of ARDS [] |
