類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Tryptase (TPS)
TPSAB1; Tryptase Alpha/Beta 1; Tryptase Alpha II; Tryptase Beta I; Tryptase-I; Tryptase-II; Tryptase-III
- 編號USEB070Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍1.56-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.59ng/mL.
- 樣本類型serum, plasma, tissue homogenates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1596 ¥ 2280 ¥ 10260 ¥ 19380 ¥ 159600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
本試劑盒用于檢測類胰蛋白酶(TPS)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將類胰蛋白酶(TPS)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的類胰蛋白酶(TPS)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的類胰蛋白酶(TPS)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的類胰蛋白酶(TPS)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPB070Ca01 | 類胰蛋白酶(TPS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPB070Ca02 | 類胰蛋白酶(TPS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAB070Ca03 | 類胰蛋白酶(TPS)多克隆抗體 | WB |
| UPAB070Ca02 | 類胰蛋白酶(TPS)多克隆抗體 | WB |
| UPAB070Ca01 | 類胰蛋白酶(TPS)多克隆抗體 | WB; IHC |
| ULAB070Ca71 | 類胰蛋白酶(TPS)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UMAB070Ca21 | 類胰蛋白酶(TPS)單克隆抗體 | WB |
| USEB070Ca | 類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEB070Ca | 類胰蛋白酶(TPS)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMB070Ca | 類胰蛋白酶(TPS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Allergy and Clinical Immunology | Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps [PubMed: 22534535] |
| J Pediatr. | Improved liver function and relieved pruritus after 4-phenylbutyrate therapy in a patient with progressive familial intrahepatic cholestasis type 2 [Pubmed:24530123] |
| Mediators of Inflammation | Mediators of Mast Cells in Bullous Pemphigoid and Dermatitis Herpetiformis [Pubmed:25400334] |
| PLOS ONE | A Novel Model of IgE-Mediated Passive Pulmonary Anaphylaxis in Rats [Pubmed:25541997] |
| Neurogastroenterology & Motility | Peritoneal mast cell degranulation and gastrointestinal recovery in patients undergoing colorectal surgery [Pubmed:25677271] |
| Mediators Inflamm | Elevated Serum Tryptase and Endothelin in Patients with ST Segment Elevation Myocardial Infarction: Preliminary Report [PubMed: 26089601] |
| Int Immunopharmacol | Tryptase and protease-activated receptor-2 stimulate scratching behavior in a murine model of ovalbumin-induced atopic-like dermatitis [PubMed: 26049029] |
| Mediators of Inflammation | Altered expression of IFN-λ2 in allergic airway disorders and identification of its cell origins [Journals: Mi] |
| Mediators of Inflammation | Altered Expression of IFN-2 in Allergic Airway Disorders and Identification of Its Cell Origins [Pubmed:27057098] |
| Analytica Chimica Acta | An ultrasensitive electrochemical detection of tryptase using 3D macroporous reduced graphene oxide nanocomposites by one-pot electrochemical synthesis [] |
| Journal of Clinical Investigation | Dengue virus-elicited tryptase induces endothelial permeability and shock [Pubmed: 31265436] |
| Frontiers in Physiology | Mast Cells in Peritoneal Fluid From Women With Endometriosis and Their Possible Role in Modulating Sperm Function [Pubmed: 31998139] |
| Biomarkers of Allergic Asthma and their Association with Serum Parameters [] | |
| Annals of Tropical Medicine & Public Health | Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity [] |
| Front Immunol | Serum CD203c+ Extracellular Vesicle Serves as a Novel Diagnostic and Prognostic Biomarker for Succinylated Gelatin Induced Perioperative Hypersensitive?… [34650557] |
| Microbiol Spectr | Mast Cell-Derived Proteases Induce Endothelial Permeability and Vascular Damage in Severe Fever with Thrombocytopenia Syndrome [Pubmed:35612327] |
