堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- 編號USEA551Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.2pg/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測堿性成纖維細(xì)胞生長因子(FGF2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的堿性成纖維細(xì)胞生長因子(FGF2)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 89-97 | 92 |
| EDTA plasma(n=5) | 78-104 | 101 |
| heparin plasma(n=5) | 89-101 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的堿性成纖維細(xì)胞生長因子(FGF2)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中堿性成纖維細(xì)胞生長因子(FGF2)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 97-105% | 80-101% | 78-104% | 79-90% |
| EDTA plasma(n=5) | 89-104% | 89-97% | 90-97% | 86-101% |
| heparin plasma(n=5) | 80-99% | 91-98% | 99-105% | 90-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將堿性成纖維細(xì)胞生長因子(FGF2)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的堿性成纖維細(xì)胞生長因子(FGF2)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的堿性成纖維細(xì)胞生長因子(FGF2)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的堿性成纖維細(xì)胞生長因子(FGF2)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA551Mu01 | 堿性成纖維細(xì)胞生長因子(FGF2)活性蛋白 | Cell?culture;?Activity?Assays. |
| URPA551Mu02 | 堿性成纖維細(xì)胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA551Mu01 | 堿性成纖維細(xì)胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA551Mu01 | 堿性成纖維細(xì)胞生長因子(FGF2)多克隆抗體 | IHC |
| UPAA551Mu02 | 堿性成纖維細(xì)胞生長因子(FGF2)多克隆抗體 | WB; IHC; ICC; IP. |
| USEA551Mu | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UCEA551Mu | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UMEA551Mu | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA551Mu | 堿性成纖維細(xì)胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA551Mu11 | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940] |
| International Journal of Nanomedicine | Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis [PubMed: PMC3254262] |
| Archives of Animal Nutrition | Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis [Tandfonline: 644918] |
| Diabetes | Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia [Pubmed: 22266669] |
| Molecular Pharmacology | Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats [Pubmed: 22091745] |
| Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source] |
| Nanoscale Research Letters | Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. [PubMed: 22827927] |
| PLoS One | Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis [PubMed: PMC3771850] |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104] |
| Archives of Animal Nutrition | Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis [Pubmed: 23952606] |
| Journal of Rehabilitation Research & Development | Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness wounds [Pubmed: 23934870] |
| PLoS One.? | Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity [Pubmed:25426952] |
| Implant Dentistry | Effects of Hemostatic Agents on Fibroblast Cells [Lww:Source] |
| Journal of Materials Chemistry B | bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healing [Rsc:Source] |
| Archives of Animal Nutrition | Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos [Pubmed:25530495] |
| Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972] |
| Theriogenology | Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article] |
| Tumor Biology | Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Acta Biomaterialia | Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa [article:S1742706115301410] |
| 22 | Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars [Pubmed:26774564] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| BMC?Neuroscience? | 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA [pubmed:27456338] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [898221] |
| Biomedicine & Pharmacotherapy | FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesis [pubmed:28841454] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [pubmed:28052053] |
| International Journal of?Clinical?and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens [ISSN:1936-2625/IJCEP0058424] |
| Journal of Biomaterials and Tissue Engineering | The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice [10.1166/jbt.2017.1652] |
| Stem?Cells?and Development | Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration [Pubmed:29630447] |
| Neuroscience?Letters | Increased serum FGF2 levels in first-episode, drug-free patients with schizophrenia [Pubmed: 30172685] |
| Biochemistry and?Cell?Biology | Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells [Pubmed: 30359541] |
| Journal of Materials Chemistry B | Gene-activating skin substitute comprised of PLLA/POSS nanofibers and plasmid DNAs encoding ANG and bFGF promotes in vivo revascularization and?… [] |
| BMC?Ophthalmology | HOXA9 is a novel myopia risk gene [Pubmed: 30674274] |
| Applied Materials Today | Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration [] |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
| Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells [] |
| Chemico-Biological Interactions | Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factor [Pubmed: 31669319] |
| International Journal of Molecular Sciences | β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe Disease [Pubmed: 31905906] |
| Molecular Medicine Reports | Long non?coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR?590?3p under hypoxic conditions [Pubmed: 32323787] |
| Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells [] | |
| Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| Materials Science & Engineering C | Double Layer Composite Membrane for Preventing Tendon Adhesion and Promoting Tendon Healing [33812576] |
| Ratlarda deneysel kolon anastomoz ka?a?? modelindeintraperitoneal atorvastatin uygulamas?n?n anastomozka?a?? onar?m? ¨1zerine etkisi [] | |
| World J Hepatol | Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosis [Pubmed:35070009] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
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