C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for C Reactive Protein (CRP)
C-RP; PTX1; Pentraxin-Related; Pentraxin 1
- 編號(hào)USEA821Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍0.312-20ng/mL
- 靈敏度最小可檢測(cè)劑量小于等于0.129ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 945 ¥ 1350 ¥ 6075 ¥ 11475 ¥ 94500
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)C反應(yīng)蛋白(CRP)，經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的C反應(yīng)蛋白(CRP)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 90-104 | 94 |
| EDTA plasma(n=5) | 89-97 | 92 |
| heparin plasma(n=5) | 82-96 | 91 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的C反應(yīng)蛋白(CRP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線性范圍即為稀釋后樣本中C反應(yīng)蛋白(CRP)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-89% | 79-98% | 98-105% | 99-105% |
| EDTA plasma(n=5) | 82-101% | 89-98% | 93-101% | 94-101% |
| heparin plasma(n=5) | 98-105% | 93-102% | 92-99% | 91-98% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將C反應(yīng)蛋白(CRP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的C反應(yīng)蛋白(CRP)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的C反應(yīng)蛋白(CRP)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的C反應(yīng)蛋白(CRP)呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度(O.D.值)，計(jì)算樣品濃度。
贈(zèng)品
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UAPA821Hu01 | C反應(yīng)蛋白(CRP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UNPA821Hu02 | C反應(yīng)蛋白(CRP)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UNPA821Hu01 | C反應(yīng)蛋白(CRP)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA821Hu62 | C反應(yīng)蛋白(CRP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA821Hu01 | C反應(yīng)蛋白(CRP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA821Hu02 | C反應(yīng)蛋白(CRP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA821Hu61 | C反應(yīng)蛋白(CRP)活性蛋白 | Cell?culture;?Activity?Assays. |
| UPAA821Hu02 | C反應(yīng)蛋白(CRP)多克隆抗體 | WB; IHC |
| UPAA821Hu01 | C反應(yīng)蛋白(CRP)多克隆抗體 | WB; IHC; ICC/IF |
| ULAA821Hu71 | C反應(yīng)蛋白(CRP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| URAA821Hu22 | C反應(yīng)蛋白(CRP)重組抗體 | WB |
| URAA821Hu23 | C反應(yīng)蛋白(CRP)重組抗體 | WB; IHC; ICC. |
| UMAA821Hu29 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB; FCM |
| UMAA821Hu21 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB |
| UMAA821Hu26 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB |
| UMAA821Hu27 | C反應(yīng)蛋白(CRP)單克隆抗體 | IHC |
| UMAA821Hu28 | C反應(yīng)蛋白(CRP)單克隆抗體 | IHC |
| UMAA821Hu24 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB; IHC |
| UMAA821Hu25 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB; IHC |
| URAA821Hu25 | C反應(yīng)蛋白(CRP)重組抗體 | WB |
| URAA821Hu21 | C反應(yīng)蛋白(CRP)重組抗體 | WB; IHC; ICC. |
| UMAA821Hu23 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB; IHC |
| UMAA821Hu22 | C反應(yīng)蛋白(CRP)單克隆抗體 | WB; IHC |
| UFAA821Hu08 | 抗C反應(yīng)蛋白(CRP)單克隆抗體 | FCM |
| UFAA821Hu02 | 抗C反應(yīng)蛋白(CRP)單克隆抗體 | FCM |
| UFAA821Hu06 | 抗C反應(yīng)蛋白(CRP)單克隆抗體 | FCM |
| UFAA821Hu04 | 抗C反應(yīng)蛋白(CRP)單克隆抗體 | FCM |
| ULAA821Hu72 | C反應(yīng)蛋白(CRP)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UHEA821Hu | C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA821Hu | C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA821Hu | 抗C反應(yīng)蛋白抗體(Anti-CRP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCA821Hu | C反應(yīng)蛋白(CRP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA821Hu | C反應(yīng)蛋白(CRP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UPSA821Hu01 | C反應(yīng)蛋白(CRP)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| UKSA821Hu01 | C反應(yīng)蛋白(CRP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
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| Experimental?Biology?and?Medicine | Grape seed proanthocyanidin extract alleviates arsenic-induced lung damage through NF-κB signaling [Pubmed: 30869553] |
| International Journal of Inflammation | Morphological Characteristics of the Thymus and Spleen and the Subpopulation Composition of Lymphocytes in Peripheral Blood during Systemic Inflammatory?… [] |
| Journal of Central European Agriculture | Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry [] |
| Journal of Clinical Medicine | Periodontal Health and Oral Microbiota in Patients with Rheumatoid Arthritis [Pubmed: 31072030] |
| Journal of Applied Pharmaceutical Science | Effects of Eurycoma longifolia Jack on chronic cerebral hypoperfusion-induced oxidative damage and memory deficit in rats [] |
| Food & Function | Eicosapentaenoic acid in the form of phospholipid exerts superior anti-atherosclerosis effects than its triglyceride form in ApoE-/-mice [] |
| Journal of Central European Agriculture | Novel assay for the detection of CRP protein in rabbit leukocytes using flow cytometry/Nova metoda detekcie CRP proteinu na kralicich leukocytoch pomocou?… [] |
| Food and Chemical Toxicology | The potential efficacy of dietary fatty acids and fructose induced inflammation and oxidative stress on the insulin signaling and fat accumulation in mice [Pubmed: 31672515] |
| Journal of Controlled Release | Ginsenoside Rg3-loaded, reactive oxygen species-responsive polymeric nanoparticles for alleviating myocardial ischemia-reperfusion injury [Pubmed: 31783047] |
| Nutrients | Rotundic Acid Protects against Metabolic Disturbance and Improves Gut Microbiota in Type 2 Diabetes Rats [Pubmed: 31887996] |
| Food & Function | Lowering effects of fish oil supplementation on proinflammatory markers in hypertension: results from a randomized controlled trial [Pubmed: 32044905] |
| JOURNAL OF HAZARDOUS MATERIALS | Silica nanoparticles induce JNK-mediated inflammation and myocardial contractile dysfunction [Pubmed: 32036317] |
| Cell Transplant | The Efficacy of Autologous Myoblast Sheet Transplantation to Prevent Perforation After Duodenal Endoscopic Submucosal Dissection in Porcine Model [Pubmed: 33121270] |
| Nanotoxicology | miR-205/IRAK2 signaling pathway is associated with urban airborne PM2.5-induced myocardial toxicity [Pubmed: 32880512] |
| Journal of the American Society of Echocardiography | Protective Effects of Statin and Angiotensin Receptor Blocker in a Rat Model of Doxorubicin-and Trastuzumab-Induced Cardiomyopathy [Pubmed: 32778498] |
| disease markers | Association between Plasma Sestrin2 Levels and the Presence and Severity of Coronary Artery Disease [Pubmed: 32670435] |
| Scientific Reports | Integrated metabolomics and gut microbiome to the effects and mechanisms of naoxintong capsule on type 2 diabetes in rats [Pubmed: 32616735] |
| animal reproduction science | Quantifying the C-reactive protein concentrations of uterine lavage samples in postpartum dairy cows [Pubmed: 32408965] |
| ANNALS OF TRANSLATIONAL MEDICINE | Serum levels of olfactomedin 4: a biomarker for asthma control state in asthmatics [Pubmed: 32395538] |
| nutrition | Validation and adaptation of the Empirical Dietary Inflammatory Pattern (EDIP) across nations: a test case. [Pubmed: 32563770] |
| European Journal of Biology | Effects of Tempol in Lipopolysaccharide-Induced Liver Injury [] |
| Possible Role of Statins on the Inflammatory Biomarkers in Patients With Periodontal Disease: A Cross-Sectional Study [] | |
| International Journal of Oral Science | Endothelin-1 is a surrogate biomarker link severe periodontitis and endothelial dysfunction in hypertensive patients: The potential nexus [] |
| Indian Journal of Animal Research | Effect of claw disorders on haemato-biochemical parameters and acute phase protein levels in crossbred cattle [] |
| Lateral Flow Immunosensor for Ferritin Based on Dual Signal-Amplified Strategy by Rhodium Nanoparticles [] | |
| Early label-free analysis of mitochondrial redox states by Raman spectroscopy predicts septic outcomes [] | |
| МАРКЕРЫ КОСТНОГО РЕМОДЕЛИРОВАНИЯ ПРИ ЗАМЕЩЕНИИ ДЕФЕКТАТРАБЕКУЛЯРНОЙ КОСТНОЙ ТКАНИ РЕЗОРБИРУЕМЫМИ И?… [] | |
| J Transl Med | Multi-omic signatures of atherogenic dyslipidaemia: pre-clinical target identification and validation in humans [33407555] |
| INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | Use of Fourier-Transform Infrared Spectroscopy (FT-IR) for Monitoring Experimental Helicobacter pylori Infection and Related Inflammatory Response in Guinea Pig??- [33396581] |
| Sci Rep | Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309] |
| Mol Med Rep | Baicalin relieves Mycoplasma pneumoniae infection?induced lung injury through regulating microRNA?221 to inhibit the TLR4/NF?|êB signaling pathway [34109422] |
| Nutrients | Prevalence and Factors Associated with Iron Deficiency and Anemia among Residents of Urban Areas of S?o Paulo, Brazil [34072813] |
| peerj | Perfluorooctane sulfonate exerts inflammatory bowel disease-like intestinal injury in rats [33510972] |
| Biomed Pharmacother | Intravenous ferric carboxymaltose does not provide benefits in reperfused acute myocardial infarction in the rat with normal iron status [34243097] |
| Aging | Pim-2 kinase inhibits inflammation by suppressing the mTORC1 pathway in atherosclerosis [34547720] |
| Analytical and Bioanalytical Chemistry | Nets of biotin-derived gold nanoparticles as a label for the C-reactive protein immunoassay [34559243] |
| J Oleo Sci | Cardio-protective and Anti-atherosclerosis Effect of Crocetin on Vitamin D3 and HFD-induced Atherosclerosis in Rats [34615830] |
| Medicine | Early-stage serum Stanniocalcin 1 as a predictor of outcome in patients with aneurysmal subarachnoid hemorrhage [Pubmed:34941085] |
| Talanta | A rapid fluorescent aptasensor for point-of-care detection of C-reactive protein [Pubmed:35714415] |
| Experimental and Therapeutic Medicine | Construction of a rabbit model with vinorelbine administration via peripherally inserted central catheter and dynamic monitoring of changes in phlebitis and thrombosis [Pubmed:35126715] |
| Sensors and Actuators B: Chemical | A Two-step Competition Assay for Visual, Sensitive and Quantitative C-reactive Protein Detection in Low-cost Microfluidic Particle Accumulators [] |
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