內(nèi)皮型一氧化氮合酶(eNOS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Endothelial NOS (eNOS)
NOS3; ECNOS; NOS III; E-NOS; C-NOS; ENOS; CNOS; Constitutive NOS; Nitric Oxide Synthase 3, Endothelial
- 編號USEA868Ra
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍1.56-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.65ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測內(nèi)皮型一氧化氮合酶(eNOS)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的內(nèi)皮型一氧化氮合酶(eNOS)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 96-105 | 101 |
| EDTA plasma(n=5) | 88-97 | 92 |
| heparin plasma(n=5) | 86-101 | 97 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的內(nèi)皮型一氧化氮合酶(eNOS)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中內(nèi)皮型一氧化氮合酶(eNOS)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-95% | 78-96% | 80-98% | 80-94% |
| EDTA plasma(n=5) | 88-97% | 84-99% | 89-101% | 80-103% |
| heparin plasma(n=5) | 91-99% | 84-94% | 87-96% | 91-103% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將內(nèi)皮型一氧化氮合酶(eNOS)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的內(nèi)皮型一氧化氮合酶(eNOS)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的內(nèi)皮型一氧化氮合酶(eNOS)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的內(nèi)皮型一氧化氮合酶(eNOS)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA868Ra01 | 內(nèi)皮型一氧化氮合酶(eNOS)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA868Ra01 | 內(nèi)皮型一氧化氮合酶(eNOS)多克隆抗體 | WB |
| ULAA868Ra71 | 內(nèi)皮型一氧化氮合酶(eNOS)多克隆抗體(生物素標(biāo)記) | WB |
| UMAA868Ra21 | 內(nèi)皮型一氧化氮合酶(eNOS)單克隆抗體 | WB; IHC; ICC; IP. |
| USEA868Ra | 內(nèi)皮型一氧化氮合酶(eNOS)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA868Ra | 內(nèi)皮型一氧化氮合酶(eNOS)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Ethnopharmacology | Impact of matrine on inflammation related factors in rat intestinal microvascular endothelial cells [ScienceDirect: S0378874109004541] |
| PLoS One | A Novel Mechanism of Formaldehyde Neurotoxicity: Inhibition of Hydrogen Sulfide Generation by Promoting Overproduction of Nitric Oxide [PubMed: 23359814] |
| 6th International Scientific Interdisciplinary Conference (ISIC) | Inflammation influence on the formation of endothelial dysfunction in hypertensive patients caused by obesity [Knmu: Source] |
| PLoS One. | SIRT1 Gene Polymorphisms Affect the Protein Expression in Cardiovascular Diseases [Plos: Source] |
| INTERNATIONAL JOURNAL OF MEDICINE AND MEDICAL RESEARCH | L-ARGININE, BUT NOT L-NAME PROTECTS AGAINST LIVER INJURY INDUCED BY EXPERIMENTAL ISCHEMIA-REPERFUSION [Tdmu:Source] |
| Journal of Clinical Laboratory Analysis | Soluble Lectin-Like Oxidized LDL Receptor 1 as a Possible Mediator of Endothelial Dysfunction in Patients With Metabolic Syndrome [Pubmed:24798146] |
| Endocrine | Chemerin plays a protective role by regulating human umbilical vein endothelial cell-induced nitric oxide signaling in preeclampsia [Pubmed:24840719] |
| PLOS ONE | A Remarkable Age-Related Increase in SIRT1 Protein Expression against Oxidative Stress in Elderly: [PubMed: 25785999] |
| J Trace Elem Med Biol | Comparative angioprotective effects of magnesium compounds [PubMed: 25127069] |
| Journal of Functional Foods | Peptides from hydrolysate of lantern fish (Benthosema pterotum) proved neuroprotective in vitro and in vivo [science:S1756464616300639] |
| Медицина и здравоохранение | Роль индуцибельной NO-синтазы в механизмах регуляции функциональной активности кальций-активируемых калиевых каналов коронарных сосудов у крыс, адаптированных к стрессу [article:n] |
| journal of molecular and cellular cardiology | Mutant DD genotype of NFKB1 gene is associated with the susceptibility and severity of coronary artery disease. [pubmed:28088561] |
| International Journal of Medicine and Medical Research | ThE REmOdELINg Of LEfT ATRIUm ANd vAsOdILATION fAcTORs chANgEs IN IschEmIc hEART fAILURE [7038] |
| International Journal of Ophthalmology | Endothelial?nitric?oxide?synthase?deficiency?influences?normal?cell?cycle?progression?andapoptosis?in?trabecular?meshwork?cells. [pubmed:27366677] |
| Biomed Pharmacother. | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death. [pubmed:28122311] |
| Biomedicine & Pharmacotherapy | SIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death [DOI:10.1016/j.biopha.2017.01.087] |
| Asian Journal of Science and Technology | THE INFLUENCE OF THE ADDITIONAL USAGE OF L-ARGININE ON THE FACTORS OF VASODILATION AND THE CLINICAL DATA, COMPLICATED WITH CARDIAC DECOMPENSATION WITH REDUCED AND PRESERVED FRACTURE OF THE LEFT VENTRICLE THAT PATIENTS WITH STABLE CORONARY HEART DISEASE HAVE [ISSN: 0976-3376] |
| National Science Review | Blunted Nitric Oxide Regulation in Tibetans under High Altitude Hypoxia [10.1093:nsr] |
| Biomedicine & Pharmacotherapy | Molecular basis of vascular damage caused by cigarette smoke exposure and a new approach to the treatment: Alpha-linolenic acid [Pubmed:29579706] |
| Experimental and Therapeutic?Medicine | Ergothioneine prevents endothelial dysfunction induced by mercury chloride [Pubmed:29805489] |
| Turk Kardiyol Dern Ars.? | Effect of statins on sirtuin 1 and endothelial nitric oxide synthase expression in young patients with a history of premature myocardial infarction [Pubmed:29664427] |
| St??enia ?ródb?onkowej i indukowalnej formy syntazy tlenku azotu w surowicy krwi kobiet ci??arnych pal?cych tytoń–badania wst?pne [] | |
| The?Eurasian?Journal of Medicine | Tadalafil Preserves Penile Nitric Oxide Synthase from Detrimental Effect of Paroxetine in Rats [Doi: 10.5152/eurasianjmed.2018.18160] |
| International Journal of Environmental Research and?Public?Health | Influence of Active Exposure to Tobacco Smoke on Nitric Oxide Status of Pregnant Women [Pubmed: 30513890] |
| Journal?of?Physics?D: Applied?Physics | Premature Myocardial Infarction: Genetic Variations in SIRT1 Affect Disease Susceptibility [] |
| Particle and?Fibre?Toxicology | Silica nanoparticles trigger the vascular endothelial dysfunction and prethrombotic state via miR-451 directly regulating the IL6R signaling pathway [Pubmed: 30975181] |
| ВЛИЯНИЕ ИНДУЦИБЕЛЬНОЙ NO-СИНТАЗЫ НА ТОНУС КОРОНАРНЫХ СОСУДОВ, СОКРАТИТЕЛЬНУЮ ФУНКЦИЮ МИОКАРДА И МЕХАНИЗМЫ?… [] | |
| Динамика показателей эндотелиальной дисфункции до и после операции у больных варикозной болезнью нижних конечностей [] | |
| European Journal of?Pharmacology | The placental growth factor attenuates intimal hyperplasia in vein grafts by improving endothelial dysfunction [Pubmed: 31836533] |
| Rheumatol Int | Circadian fluctuations of endothelial nitric oxide synthase activity in females with rheumatoid arthritis: a pilot study [Pubmed: 32025851] |
| Reviews on Recent Clinical Trials | Circadian rhythms of endothelial nitric oxide synthase and toll-like receptors 2 production in females with rheumatoid arthritis depending on NOS3 gene polymorphism?… [Pubmed: 32297585] |
| Georgian medical news | ASSOCIATIONS BETWEEN EFFICACY OF THE THERAPY AND CIRCADIAN FLUCTUATIONS OF ENDOTHELIAL NITRIC OXIDE SYNTHASE WITH TOLL-LIKE?… [Pubmed: 32242853] |
| Zaporozhye medical journal | Dynamics of endothelial dysfunction indices before and after operation in patients with varicose disease of the lower extremities [] |
| regional hemodynamics and microcirculation | Benzimidazole Derivative, 2-ETH Prevents Poststressor Disorders of Coronary Vascular Tone and Myocardial Contractility [] |
| Оксидативный стресс и эндотелиальная дисфункция в развитии экспериментального радиоиндуцированного повреждения легких и их коррекция?… [] | |
| Reprod Biol | Angiogenic factors measured in aspirated placental tissue between the 10+ 6 and 18+ 3 weeks of gestation [34798517] |
| J Basic Clin Physiol Pharmacol | Anti-atherosclerotic activity of aqueous extract of Ipomoea batatas (L.) leaves in high-fat diet-induced atherosclerosis model rats [Pubmed:34986543] |
| Eur Rev Med Pharmacol Sci | Serum inducible and endothelial nitric oxide synthase in coronary artery disease patients with Type 2 Diabetes mellitus [Pubmed:35647851] |
| Evidence-Based Complementary and Alternative Medicine | Mechanism of Fructus Mume Pills Underlying Their Protective Effects in Rats with Acetic Acid-Inducedulcerative Colitis via the Regulation of Inflammatory?… [Pubmed:35620404] |
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