分泌型免疫球蛋白A(sIgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Secretory Immunoglobulin A (sIgA)
S-IgA
- 編號UCEA641Rb
- 物種Oryctolagus cuniculus (Rabbit，兔) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應(yīng)時長2h
- 檢測范圍617.3-50,000ng/mL
- 靈敏度最小可檢測劑量小于等于241.3ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1436 ¥ 2052 ¥ 9234 ¥ 17442 ¥ 143640
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測分泌型免疫球蛋白A(sIgA)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的分泌型免疫球蛋白A(sIgA)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-88 | 83 |
| EDTA plasma(n=5) | 78-89 | 85 |
| heparin plasma(n=5) | 89-97 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的分泌型免疫球蛋白A(sIgA)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中分泌型免疫球蛋白A(sIgA)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 86-101% | 83-93% | 81-103% | 90-98% |
| EDTA plasma(n=5) | 78-96% | 89-99% | 96-104% | 85-94% |
| heparin plasma(n=5) | 81-94% | 96-105% | 84-98% | 81-90% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將分泌型免疫球蛋白A(sIgA)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
相關(guān)產(chǎn)品
| 編號 | 適用物種：Oryctolagus cuniculus (Rabbit，兔) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UCEA641Rb | 分泌型免疫球蛋白A(sIgA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA641Rb | 分泌型免疫球蛋白A(sIgA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| British Journal of Nutrition | Evaluation of the immune benefits of two probiotic strains Bifidobacterium animalis ssp. lactis, BB-12? and Lactobacillus paracasei ssp. paracasei, L. casei 431? in an influenza vaccination model: a randomised, double-blind, placebo-controlled study. [Solgar: source] |
| Jornal teknologi dan industri pangan | RESPON IMUN MUKOSA DAN SELULER PADA TIKUS YANG DIBERI BUBUK SUSU KAMBING DENGAN INFEKSI Salmonella Typhimurium [Mucosal and Cellular Immune Response of Rat Given Goat Milk Powder and Infected with Salmonella Typhimurium] [Ipb: Source] |
| Poultry Science | Effects of Clostridium butyricum on growth performance, immune function, and cecal microflora in broiler chickens challenged with Escherichia coli K88 [Pubmed: 24570422 ] |
| Journal of Food Research | Potency of Lactobacillus plantarum Dad-13 and Sweet Potato (Ipomoea batatas) Fiber as Immunomodulator in Rats Infected With Salmonella Typhimurium [Ccsenet:Source] |
| PLoS One. | Repeated Oronasal Exposure to Lipopolysaccharide Induced Mucosal IgA Responses in Periparturient Dairy Cows [Pubmed:25061754] |
| Bioscience, Biotechnology, and Biochemistry | Dietary Apostichopus japonicus enhances the respiratory and intestinal mucosal immunity in immunosuppressive mice [Pubmed:25186733] |
| Food & Function | Dietary squid ink polysaccharide could enhance SIgA secretion in chemotherapeutic mice [Pubmed:25308407] |
| Front Microbiol | Fecal microbiota transplantation and bacterial consortium transplantation have comparable effects on the re-establishment of mucosal barrier function in mice with intestinal dysbiosis [PubMed: 26217323] |
| J Hum Lact. | Role of Maternal Allergy on Immune Markers in Colostrum and Secretory Immunoglobulin A in Stools of Breastfed Infants [PubMed: 26243755] |
| Pediatrics | Oropharyngeal Colostrum Administration in Extremely Premature Infants: An RCT [PubMed: 25624376] |
| Biomed Res Int. | Salivary Secretory Immunoglobulin (SIgA) and Lysozyme in Malignant Tumor Patients [journals:8701423] |
| 動物營養(yǎng)學(xué)報 | 益生菌抵御大鼠急性腹瀉的效果及其對腸道黏膜屏障的影響 [article:12422] |
| Sci Rep.? | Long-term use of ceftriaxone sodium induced changes in gut microbiota and immune system. [pubmed:28220870] |
| Acta Vet Hung. | Imbalance of intestinal immune function in piglets infected by porcine circovirus type 2 during the fetal period. [pubmed:28244338] |
| Food & Function | Immunomodulatory effects of Hericium erinaceus derived polysaccharides are mediated by intestinal immunology [pubmed:28266682] |
| Pathogens and Disease | Saccharomyces boulardiiadministration can inhibit the formation of gastric lymphoid follicles induced by?Helicobacter suis?infection [pubmed:28115360] |
| poultry?science | Effects of dietary threonine supplementation on intestinal barrier function and gut microbiota of laying hens [pubmed:28938780] |
| Journal of Functional Foods | Novel dietary polysaccharide SIP promotes intestinal secretory immunoglobulin A secretion in mice under chemotherapy [10.1016/j.jff.2017.08.012] |
| International Journal of Pharmacology | Evaluation of Susceptibility and Innate Immune Response to Candida albicans in Mice with Sub-health [ijp.2018.689.697] |
| Journal of Dental Research | Correlation of s-IgA and IL-6 Salivary with Caries Disease and Oral Hygiene Parameters in Children [Pubmed: 31892186] |
| Analytical Chemistry | Fluorescence polarization-based rapid detection system for salivary biomarkers using modified DNA aptamers containing base-appended bases [Pubmed: 31855403] |
| Eur J Pharmacol | Artesunate protects immunosuppression mice induced by glucocorticoids via enhancing pro-inflammatory cytokines release and bacterial clearance [Pubmed: 33045197] |
| RESEARCH IN VETERINARY SCIENCE | Effects of different doses lipopolysaccharides on the mucosal barrier in mouse intestine [Pubmed: 32947071] |
| JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis [Pubmed: 32567144] |
| Vaccines | Construction and Immunogenicity Comparison of Three Virus-Like Particles Carrying Different Combinations of Structural Proteins of Avian Coronavirus Infectious??- [33670249] |
| Nutrients | Ingestion of High |?-Glucan Barley Flour Enhances the Intestinal Immune System of Diet-Induced Obese Mice by Prebiotic Effects [33799564] |
| J Orofac Orthop | Assessment of salivary stress and pain biomarkers and their relation to self-reported pain intensity during orthodontic tooth movement: a longitudinal and prospective?… [34170330] |
| Nutrients | Microarray Analysis of Paramylon, Isolated from Euglena Gracilis EOD-1, and Its Effects on Lipid Metabolism in the Ileum and Liver in Diet-Induced Obese Mice [34684408] |
| Medicine (Baltimore) | Saliva testing as noninvasive way for monitoring exercise-dependent response in teenage elite water polo players: A cohort study [34797320] |
| Animals | Fermented Corn–Soybean Meal Mixed Feed Modulates Intestinal Morphology, Barrier Functions and Cecal Microbiota in Laying Hens [34827791] |
| Food Funct | Three Urban of China: A Cohort Study of Maternal-Infant Factors and HM Protein Components [Pubmed:35438093] |
