血管細(xì)胞粘附分子1(VCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Vascular Cell Adhesion Molecule 1 (VCAM1)
CD106; INCAM-100; L1CAM
- 編號USEA547Ca
- 物種Canis familiaris; Canine (Dog，犬) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍3.12-200ng/mL
- 靈敏度最小可檢測劑量小于等于1.25ng/mL.
- 樣本類型Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 1512 ¥ 2160 ¥ 9720 ¥ 18360 ¥ 151200
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結(jié)果圖
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標(biāo)準(zhǔn)曲線圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測血管細(xì)胞粘附分子1(VCAM1)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的血管細(xì)胞粘附分子1(VCAM1)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-89 | 86 |
| EDTA plasma(n=5) | 78-101 | 95 |
| heparin plasma(n=5) | 85-103 | 93 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的血管細(xì)胞粘附分子1(VCAM1)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中血管細(xì)胞粘附分子1(VCAM1)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-90% | 81-99% | 93-101% | 91-105% |
| EDTA plasma(n=5) | 94-102% | 83-94% | 81-97% | 92-101% |
| heparin plasma(n=5) | 93-102% | 95-102% | 87-101% | 86-94% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將血管細(xì)胞粘附分子1(VCAM1)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的血管細(xì)胞粘附分子1(VCAM1)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的血管細(xì)胞粘附分子1(VCAM1)抗體，將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的血管細(xì)胞粘附分子1(VCAM1)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Canis familiaris; Canine (Dog，犬) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA547Ca01 | 血管細(xì)胞粘附分子1(VCAM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA547Ca01 | 血管細(xì)胞粘附分子1(VCAM1)多克隆抗體 | WB |
| USEA547Ca | 血管細(xì)胞粘附分子1(VCAM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA547Ca | 血管細(xì)胞粘附分子1(VCAM1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Amran et al. Lipids in Health and Disease | Changes in the vascular cell adhesion molecule-1, intercellular adhesion molecule-1 and c-reactive protein following administration of aqueous extract of piper sarmentosum on experimental. [BioMed: 1476511X] |
| Vascular Pharmacology | Long-term ethanol consumption initiates atherosclerosis in rat aorta through inflammatory stress and endothelial dysfunction [ScienceDirect: S1537189112000705] |
| Canadian Journal of Physiology and Pharmacology | Resveratrol abrogates adhesion molecules and protects against TNBS-induced ulcerative colitis in rats. [PubMed: 22029500] |
| British Journal of Medical and Health Sciences | EFFECTS OF ADRIAMYCIN, CISPLATIN, AND 5-FLUOROURACIL ON THE TESTES OF ALBINO RATS [Researchgate: Source] |
| Endocrine Metabolic & Immune Disordors Drug Targets. | Active immunization against tumor necrosis factor-alpha decreases proinflammatory cytokines, oxidative stress mediators and adhesion molecules risk factors in streptozotocin-induced diabetic rats. [Pubmed: 23859636] |
| Atherosclerosis | Increased monocyte adhesion by endothelial expression of VCAM-1 missense variation in vitro [Pubmed: 24075742] |
| Atherosclerosis | Activation of receptor for advanced glycation end products contributes to aortic remodeling and endothelial dysfunction in sinoaortic denervated rats. [Pubmed: 23880178] |
| BioMed research international | Endothelial gene expression and molecular changes in response to radiosurgery in in vitro and in vivo models of cerebral arteriovenous malformations [Pubmed: 24199192] |
| Arch Dis Child | PO-0699 Estimation Of Adhesion Molecules Intrauterine Growth Retardation (iugr) Newborns [Bmj:Source] |
| Arch Dis Child? | PO-0701 Causes Of Fetal Growth Restriction And Early Neonatal Outcome [Bmj:Source] |
| Arch Dis Child | PO-0698 Neonatal Resuscitation Practices In The Delivery Room In India: An Audit Using Videography [Bmj:Source] |
| Naunyn-Schmiedeberg's Archives of Pharmacology | Insights into the antiatherogenic molecular mechanisms of andrographolide against?Porphyromonas gingivalis-induced atherosclerosis in rabbits [Pubmed:25172523] |
| Nutr Res. | Curcumin attenuates adhesion molecules and matrix metalloproteinase expression in hypercholesterolemic rabbits [Pubmed:25282128] |
| Alexandria Journal of Medicine | Effect of consumption of fresh and heated virgin coconut oil on the blood pressure and inflammatory biomarkers: An experimental study in Sprague Dawley rats [Sciencedirect:S2090506814000268] |
| Canadian Journal of Physiology and Pharmacology | Effect of magnesium sulfate and thyroxine on inflammatory markers in a rat model of hypothyroidism [Doi: Abs] |
| Hum Exp Toxicol | Effect of agmatine on experimental vascular endothelial dysfunction [PubMed: 26424770] |
| International Journal of Food Sciences and Nutrition | Serum lipid profile and inflammatory markers in the aorta of cholesterol-fed rats supplemented with extra virgin olive oil, sunflower oils and oil-products [PubMed: 26401576] |
| J Trace Elem Med Biol | Comparative angioprotective effects of magnesium compounds [PubMed: 25127069] |
| J Mol Cell Cardiol | Diverse contribution of bone marrow-derived late-outgrowth endothelial progenitor cells to vascular repair under pulmonary arterial hypertension and arterial neointimal formation [PubMed: 26231083] |
| Endocrine | Effects of glucagon-like peptide-1 on advanced glycation endproduct-induced aortic endothelial dysfunction in streptozotocin-induced diabetic rats: possible roles of Rho kinase- and AMP kinase-mediated nuclear factor κB signaling pathways [Pubmed:26758998] |
| Mol Neurobiol | A Therapeutic Insight of Niacin and Coenzyme Q10 Against Diabetic Encephalopathy in Rats [Pubmed:26867655] |
| Pakistan journal of pharmaceutical sciences | Changes in blood pressure, vascular reactivity and inflammatory biomarkers following consumption of heated corn oil. [pubmed:29084680] |
| Acta Biochim Biophys Sin | Therapeutic potential of mesenchymal stem cells in acute kidney injury is affected by administration timing [pubmed:28338909] |
| Experimental and Therapeutic Medicine | Epigallocatechin?3?gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression [pubmed:28962112] |
| Experimental Parasitology | Adhesion molecules, chemokines and matrix metallo-proteinases response after albendazole and albendazole plus steroid therapy in swine neurocysticercosis [pubmed:28821422] |
| Evidence-Based Complementary and Alternative Medicine | BuShenKangShuai tablet reduces atherosclerotic lesion by improving blood lipids metabolism and inhibiting inflammatory response via TLR4 and NF-κB signaling pathway [journals:1758383] |
| Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
| International Journal of?Clinical?and Experimental Pathology | Protective effect of high mobility group box-1 silence on diabetic retinopathy: an in vivo study [ISSN:1936-2625/IJCEP0055881] |
| ResearchGate | Significance of Soluble Endoglin as a Possible Marker of Cardiovascular Disease in Type 2 Diabetic Patients [315113405] |
| Regenerative Biomaterials | The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic [10.1093/rb/rbx028] |
| Anesthesiology | Vasculotide, an angiopoietin-1 mimetic, restores microcirculatory perfusion and microvascular leakage and decreases fluid resuscitation requirements in?… [Pubmed:28968277] |
| PLoS Neglected Tropical Diseases | Characterization of the rhesus macaque (Macaca mulatta) scrub typhus model: Susceptibility to intradermal challenge with the human pathogen Orientia?… [Pubmed:29522521] |
| Life Sciences | Nephroprotective effect of saxagliptin against gentamicin-induced nephrotoxicity, emphasis on anti-oxidant, anti-inflammatory and anti-apoptic effects [Pubmed:30012474] |
| Evidence-based?Complementary and Alternative?Medicine | Bushenkangshuai Tablet Reduces Atherosclerotic Lesion by Improving Blood Lipids Metabolism and Inhibiting Inflammatory Response via TLR4 and NF-κB Signaling?… [Pubmed:29619063] |
| Nutrition?&?Metabolism | L-Carnitine Supplementation Increases Trimethylamine-N-Oxide but not Markers of Atherosclerosis in Healthy Aged Women [Pubmed: 30485835] |
| Cytokine | Intercellular adhesion molecule-1 (ICAM-1) associates with 24-hour ambulatory blood pressure variability in type 2 diabetes and controls [Pubmed: 30716657] |
| Anticancer?Research | Effect of Radiation on the Expression of CVD-related miRNAs, Inflammation and Endothelial Dysfunction of HUVECs [Pubmed: 30711956] |
| European?Journal of Pharmacology | The cordycepin derivative IMM-H007 improves endothelial dysfunction by suppressing vascular inflammation and promoting AMPK-dependent eNOS activation in?… [Pubmed: 30826323] |
| international journal of molecular sciences | Anti-Inflammatory and Anti-Apoptotic Effects of Stybenpropol A on Human Umbilical Vein Endothelial Cells [Pubmed: 31671764] |
| Archives of Insect Biochemistry and Physiology | Vanadyl sulphate ameliorates biomarkers of endothelial injury and coagulation and thrombosis in a rat model of hyperglycaemia [Pubmed: 31774317] |
| BMC Endocrine Disorders | The role of the adipocytokines vaspin and visfatin in vascular endothelial function and insulin resistance in obese children [Pubmed: 31771561] |
| Scientific Reports | Association between Upper-airway Surgery and Ameliorative Risk Markers of endothelial function in obstructive Sleep Apnea [Pubmed: 31882827] |
| BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE | Endothelial epithelial sodium channel involves in high-fat diet-induced atherosclerosis in low-density lipoprotein receptor-deficient mice [Pubmed: 33065235] |
| Antioxidants (Basel) | Anti-Atherogenic Effects of Orlistat on Obesity-Induced Vascular Oxidative Stress Rat Model [33562069] |
| Sci. Pharm | Dehydroepiandrosterone (DHEA) Improves the Metabolic and Haemostatic Disturbances in Rats with Male Hypogonadism [] |
| Journal of the South African Veterinary Association | Circulating markers of endothelial activation in canine parvoviral enteritis [] |
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