基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)
Instant ELISA Kit for Matrix Metalloproteinase 9 (MMP9)
GELB; Gelatinase B; CLG4B; CLG4-B; 92 KDa Gelatinase; 92kDa Type IV Collagenase
- 編號UIEA553Mu
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長1h, 10min
- 檢測范圍-
- 靈敏度-
- 樣本類型-
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 82-98 | 91 |
| EDTA plasma(n=5) | 90-104 | 96 |
| heparin plasma(n=5) | 80-104 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 79-93% | 81-101% | 91-99% | 95-103% |
| EDTA plasma(n=5) | 88-97% | 79-94% | 81-94% | 81-98% |
| heparin plasma(n=5) | 79-93% | 92-99% | 85-98% | 80-90% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C溫育30分鐘;
3. 甩干，加檢測溶液A100µL，37°C溫育30分鐘;
4. 洗板3次；
5. 加檢測溶液B100µL， 37°C孵育10分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10－20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)單克隆抗體包被于96孔微孔板中，制成固相載體，向微孔中加入標(biāo)準(zhǔn)品或標(biāo)本以及同時(shí)加入HRP標(biāo)記基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型)的多克隆抗體，孵育結(jié)束徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的被檢測物濃度呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計(jì)算樣品濃度。
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| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| UEPA553Mu63 | 基質(zhì)金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA553Mu51 | 基質(zhì)金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UAPA553Mu01 | 基質(zhì)金屬蛋白酶9(MMP9)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA553Mu61 | 基質(zhì)金屬蛋白酶9(MMP9)活性蛋白 | Cell?culture;?Activity?Assays. |
| UAPA553Mu62 | 基質(zhì)金屬蛋白酶9(MMP9)活性蛋白 | Cell?culture;?Activity?Assays. |
| UEPA553Mu62 | 基質(zhì)金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UEPA553Mu61 | 基質(zhì)金屬蛋白酶9(MMP9)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA553Mu01 | 基質(zhì)金屬蛋白酶9(MMP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA553Mu02 | 基質(zhì)金屬蛋白酶9(MMP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA553Mu02 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體 | WB; IHC |
| UPAA553Mu01 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體 | WB; ICC/IF |
| UPAA553Mu07 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體 | IHC |
| ULAA553Mu71 | 基質(zhì)金屬蛋白酶9(MMP9)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| UIEA553Mu | 基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| USEA553Mu | 基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UAEA553Mu | 抗基質(zhì)金屬蛋白酶9抗體(Anti-MMP9)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| USCA553Mu | 基質(zhì)金屬蛋白酶9(MMP9)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| ULMA553Mu | 基質(zhì)金屬蛋白酶9(MMP9)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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| Medicina | Proportion of the CD19-Positive and CD19-Negative Lymphocytes and Monocytes within the Peripheral Blood Mononuclear Cell Set Is Characteristic for Rheumatoid … [Pubmed: 31554310] |
| Oncology Research | MicroRNA-6884-5p regulates the proliferation, invasion and EMT of gastric cancer cells by directly targeting S100A16 [Pubmed: 31796150] |
| Open Medicine | Plasma matrix metalloproteinase-9 and tissue inhibitor of matrix metalloproteinase-1 as prognostic biomarkers in critically ill patients [Pubmed: 32190734] |
| Scientific Reports | Matrix metallopeptidase expression and modulation by transforming growth factor-β1 in equine endometrosis [Pubmed: 31980722] |
| JOURNAL OF THROMBOSIS AND THROMBOLYSIS | Extracellular matrix collagen biomarkers levels in patients with chronic thromboembolic pulmonary hypertension [Pubmed: 33175289] |
| Eur J Pharmacol | TLR3 inhibitor and tyrosine kinase inhibitor attenuate cigarette smoke/poly I: C-induced airway inflammation and remodeling by the EGFR/TLR3/MAPK?… [Pubmed: 33068589] |
| Cancers | Multiple Myeloma-Derived Extracellular Vesicles Induce Osteoclastogenesis through the Activation of the XBP1/IRE1α Axis [Pubmed: 32759820] |
| Lab Invest | Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis [Pubmed: 32709888] |
| International Journal of Environmental Research and Public Health | Biomarkers Facilitate the Assessment of Prognosis in Critically Ill Patients with Primary Brain Injury: A Cohort Study [Pubmed: 32575870] |
| J Cell Physiol | A novel role of VEPH1 in regulating AoSMC phenotypic switching [Pubmed: 32342520] |
| JAMA Surgery | Intraoperative dexmedetomidine for prevention of postoperative delirium in elderly patients with mild cognitive impairment [Pubmed: 28593326] |
| OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY | Intracranial Aneurysm Biomarker Candidates Identified by a Proteome-Wide Study [Pubmed: 32525733] |
| Advances in Animal and Veterinary Sciences | Clinicopathological studies and new markers for trypanosoma evansi in experimen-tally infected rats [] |
| International Journal of Pharmacology | Anti-arthritic Effects of Platelets Rich Plasma and Hyaluronic Acid on Adjuvant-induced Arthritis in Rats [] |
| Asian Journal of Pharmacy and Pharmacology | Evaluation of altered ground matrix and matrix metalloproeinase (MMP'S) in wound healing with Aloe vera extract [] |
| Veterinary Sciences: Research and Reviews | Investigation of matrix metalloproteinase-2 and matrix metalloproteinase-9 activity in some inflammatory conditions in Barki sheep [] |
| Identification of Polyphenolic Compounds and Hepatoprotective Activity of Artichoke (Cynara Scolymus L.) Edible Part Extracts in Rats [] | |
| Клиническая медицина | ДИНАМИКА МАРКЕРА ФИБРОЗИРОВАНИЯ ММР-9 У БОЛЬНЫХ ЛИМФОМОЙ ХОДЖКИНА [] |
| ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH | Krill oil and low-dose aspirin combination mitigates experimentally induced silicosis in rats: role of NF-|êB/TGF-|?1/MMP-9 pathway [33398741] |
| Oncol Lett | GATA3 is downregulated in HCC and accelerates HCC aggressiveness by transcriptionally inhibiting slug expression [33613720] |
| CLINICAL SCIENCE | Berberine accelerated wound healing by restoring TrxR1/JNK in diabetes [33491733] |
| Sci Rep | Association between MIF gene promoter rs755622 and susceptibility to coronary artery disease and inflammatory cytokines in the Chinese Han population [33850223] |
| MOLECULAR VISION | Quantification of a panel for dry-eye protein biomarkers in tears: A comparative pilot study using standard ELISA and customized microarrays [34012227] |
| Journal of Pregnancy | Matrix Metalloproteinases MMP-2 and MMP-9 Occupy a New Role in Severe Preeclampsia [33381317] |
| Stem Cells International | The Paracrine Effect of Adipose-Derived Stem Cells Orchestrates Competition between Different Damaged Dermal Fibroblasts to Repair UVB-Induced Skin??- [33381190] |
| DOCSBAY | Intermittent Pacing Therapy Favorably Modulates Infarct Remodeling [28386775] |
| Drug Chem Toxicol | Study of cardioprotective activity of the methanolic extract of the aerial parts of Bauhinia madagascariensis compared to Bauhinia purpurea against adrenaline?… [34167392] |
| JOURNAL OF FLUORESCENCE | Time-resolved Fluorescence Immunoassay (TRFIA) for the Simultaneous Detection of MMP-9 and Lp-PLA2 in Serum [34495467] |
| CNS Neurosci Ther | Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke [34591349] |
| Calcium Binding Protein S100A16 Expedites Proliferation, Invasion and EMT Process in Gastric Cancer [34650982] | |
| Disease Markers | Urinary Matrix Metalloproteinase-9 and Nephrin in Idiopathic Membranous Nephropathy: A Cross-Sectional Study [34707724] |
| Medicina (Kaunas) | Plasma Concentrations of New Biochemical Markers of Atherosclerosis in Patients with Dyslipidemia—A Pilot Study [Pubmed:35743980] |
| Biomolecules | Increase in Serum MMP-9 and TIMP-1 Concentrations during Alcohol Intoxication in Adolescents—A Preliminary Study [Pubmed:35625637] |
| Journal of Pharmaceutical Research International | Concomitant Administration of Rosuvastatin and Lefleunamide in Low doses Synergize Against Complete Freund's Adjuvant (CFA)-Induced Rheumatoid?… [] |
| Non-POU Domain-Containing Octamer-Binding (NONO) Protein Stability Regulated by PIN1 is Crucial for Breast Cancer Tumorigenicity Via the MAPK/β?… [] | |
| Turkish Journal of Biochemistry-Turk Biyokimya Dergisi | Downregulation of MMP-2 and MMP-9 genes in obesity patients and their relation with obesity-related phenotypes [] |
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