60kD熱休克蛋白(Hsp60)多克隆抗體
Polyclonal Antibody to Heat Shock Protein 60 (Hsp60)
HSPD1; GROEL; CPN60; HuCHA60; Heat Shock 60kD Protein 1, Chaperonin; Spastic Paraplegia 13,Autosomal Dominant; 60 kDa chaperonin; Mitochondrial matrix protein P1; P60 lymphocyte protein
- 編號UPAA822Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA822Hu02-60kD熱休克蛋白(Hsp60)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Rattus norvegicus (Rat，大鼠)
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 284 ¥ 664 ¥ 948 ¥ 2370 ¥ 9480
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Glioma Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach cancer Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Breast cancer Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 30μg/ml Rabbit Anti-Human HSPD1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: HepG2 cell lysate; Primary Ab: 0.01μg/ml Rabbit Anti-Human Hsp60 Antibody Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Samples: Lane1: MCF7 cell lysate; Lane2: A549 cell lysate;
Primary Ab: 0.01μg/ml Rabbit Anti-Human Hsp60 Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human HSPD1 Polyclonal Antibody (Catalog # PAA822Hu02, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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Human Hela cell was stained with 20μg/ml rabbit Anti-human Hsp60 Polyclonal Antibody (Catalog PAA822Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對Hsp60的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別Hsp60。
用法
Western blotting: 0.5-2μg/mL
Immunohistochemistry: 5-20μg/mL
Immunocytochemistry: 5-20μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| URPA822Hu02 | 60kD熱休克蛋白(Hsp60)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| URPA822Hu01 | 60kD熱休克蛋白(Hsp60)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| UPAA822Hu01 | 60kD熱休克蛋白(Hsp60)多克隆抗體 | WB; IHC; ICC/IF |
| UPAA822Hu02 | 60kD熱休克蛋白(Hsp60)多克隆抗體 | WB; IHC; FCM |
| UFAA822Hu01 | 抗60kD熱休克蛋白(Hsp60)多克隆抗體 | FCM |
| ULAA822Hu81 | 60kD熱休克蛋白(Hsp60)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| ULAA822Hu71 | 60kD熱休克蛋白(Hsp60)多克隆抗體(生物素標(biāo)記) | WB |
| UFAA822Hu41 | 抗60kD熱休克蛋白(Hsp60)多克隆抗體(藻紅素標(biāo)記) | FCM |
| UMAA822Hu28 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | ICC/IF |
| UMAA822Hu22 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA822Hu21 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC; ICC/IF |
| UMAA822Hu23 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; IHC |
| UMAA822Hu24 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB; ICC/IF |
| UMAA822Hu25 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB |
| UMAA822Hu27 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB |
| UMAA822Hu26 | 60kD熱休克蛋白(Hsp60)單克隆抗體 | WB |
| UFAA822Hu02 | 抗60kD熱休克蛋白(Hsp60)單克隆抗體 | FCM |
| USEA822Hu | 60kD熱休克蛋白(Hsp60)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| UHEA822Hu | 60kD熱休克蛋白(Hsp60)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| ULMA822Hu | 60kD熱休克蛋白(Hsp60)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| UKSA822Hu01 | 60kD熱休克蛋白(Hsp60)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Sci Rep | Hsp60 response in experimental and human temporal lobe epilepsy [PubMed: 25801186] |
| Cellular?and Molecular Gastroenterology and Hepatology | Senescent hepatocytes in decompensated liver show reduced UPR and its key player, CLPP, attenuates senescence in vitro [Pubmed: 30878663] |
| Breast Cancer Res Treat | Activation of mitochondrial unfolded protein response is associated with Her2-overexpression breast cancer [Pubmed: 32601970] |
